LOCUS       sequence-84302-e        2237 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytokeratin, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2237)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Keratin14-N-18
FEATURES             Location/Qualifiers
     source          1..2237
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1490..1499
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1496..1498,1499..1501,1502..2215)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1541..1562)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1802..1821)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2149..2170
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ccaccatgac tacctgcagc cgccagttca cctcctccag
       61 ctccatgaag ggctcctgcg gcatcggggg cggcatcggg ggcggctcca gccgcatctc
      121 ctccgtcctg gccggagggt cctgccgcgc ccccagcacc tacgggggcg gcctgtctgt
      181 ctcatcctcc cgcttctcct ctgggggagc ctatgggttg gggggcggct atggcggtgg
      241 cttcagcagc agcagcagca gctttggtag tggctttggg ggaggatatg gtggtggcct
      301 tggtgctggc ttgggtggtg gctttggtgg tggctttgct ggtggtgatg ggcttctggt
      361 gggcagtgag aaggtgacca tgcagaacct caacgaccgc ctggcctcct acctggacaa
      421 ggtgcgtgct ctggaggagg ccaacgccga cctggaagtg aagatccgtg actggtacca
      481 gaggcagcgg cctgctgaga tcaaagacta cagtccctac ttcaagacca ttgaggacct
      541 gaggaacaag attctcacag ccacagtgga caatgccaat gtccttctgc agattgacaa
      601 tgcccgtctg gccgcggatg acttccgcac caagtatgag acagagttga acctgcgcat
      661 gagtgtggaa gccgacatca atggcctgcg cagggtgctg gacgaactga ccctggccag
      721 agctgacctg gagatgcaga ttgagagcct gaaggaggag ctggcctacc tgaagaagaa
      781 ccacgaggag gagatgaatg ccctgagagg ccaggtgggt ggagatgtca atgtggagat
      841 ggacgctgca cctggcgtgg acctgagccg cattctgaac gagatgcgtg accagtatga
      901 gaagatggca gagaagaacc gcaaggatgc cgaggaatgg ttcttcacca agacagagga
      961 gctgaaccgc gaggtggcca ccaacagcga gctggtgcag agcggcaaga gcgagatctc
     1021 ggagctccgg cgcaccatgc agaacctgga gattgagctg cagtcccagc tcagcatgaa
     1081 agcatccctg gagaacagcc tggaggagac caaaggtcgc tactgcatgc agctggccca
     1141 gatccaggag atgattggca gcgtggagga gcagctggcc cagctccgct gcgagatgga
     1201 gcagcagaac caggagtaca agatcctgct ggacgtgaag acgcggctgg agcaggagat
     1261 cgccacctac cgccgcctgc tggagggcga ggacgcccac ctctcctcct cccagttctc
     1321 ctctggatcg cagtcatcca gagatgtgac ctcctccagc cgccaaatcc gcaccaaggt
     1381 catggatgtg cacgatggca aggtggtgtc cacccacgag caggtccttc gcaccaagaa
     1441 cagcagcggt ggagcaagcg cagccagtgg tagcgcggat ccaccggtcg ccaccatggt
     1501 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1561 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1621 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1681 gaccaccttg acctacggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     1741 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1801 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1861 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1921 ggagtacaac tacaacagcc acaaggtcta tatcaccgcc gacaagcaga agaacggcat
     1981 caaggtgaac ttcaagaccc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     2041 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     2101 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     2161 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagcggc
     2221 cgcgactcta gatcata
//
