LOCUS       sequence-84304-e        2808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2808)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-LaminA-C-18
FEATURES             Location/Qualifiers
     source          1..2808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(27..29,30..32,33..743)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(72..93)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(333..352)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     680..701
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
       61 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      121 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      181 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt gacctacggc gtgcagtgct
      241 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      301 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      361 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      421 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaaggtct
      481 atatcaccgc cgacaagcag aagaacggca tcaaggtgaa cttcaagacc cgccacaaca
      541 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      601 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc
      661 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
      721 tcggcatgga cgagctgtac aagtccggac tcagatctgg cagcggtgga ggcagcgcat
      781 ccggcggaag cggaagcgag accccgtccc agcggcgcgc cacccgcagc ggggcgcagg
      841 ccagctccac tccgctgtcg cccacccgca tcacccggct gcaggagaag gaggacctgc
      901 aggagctcaa tgatcgcttg gcggtctaca tcgaccgtgt gcgctcgctg gaaacggaga
      961 acgcagggct gcgccttcgc atcaccgagt ctgaagaggt ggtcagccgc gaggtgtccg
     1021 gcatcaaggc cgcctacgag gccgagctcg gggatgcccg caagaccctt gactcagtag
     1081 ccaaggagcg cgcccgcctg cagctggagc tgagcaaagt gcgtgaggag tttaaggagc
     1141 tgaaagcgcg caataccaag aaggagggtg acctgatagc tgctcaggct cggctgaagg
     1201 acctggaggc tctgctgaac tccaaggagg ccgcactgag cactgctctc agtgagaagc
     1261 gcacgctgga gggcgagctg catgatctgc ggggccaggt ggccaagctt gaggcagccc
     1321 taggtgaggc caagaagcaa cttcaggatg agatgctgcg gcgggtggat gctgagaaca
     1381 ggctgcagac catgaaggag gaactggact tccagaagaa catctacagt gaggagctgc
     1441 gtgagaccaa gcgccgtcat gagacccgac tggtggagat tgacaatggg aagcagcgtg
     1501 agtttgagag ccggctggcg gatgcgctgc aggaactgcg ggcccagcat gaggaccagg
     1561 tggagcagta taagaaggag ctggagaaga cttattctgc caagctggac aatgccaggc
     1621 agtctgctga gaggaacagc aacctggtgg gggctgccca cgaggagctg cagcagtcgc
     1681 gcatccgcat cgacagcctc tctgcccagc tcagccagct ccagaagcag ctggcagcca
     1741 aggaggcgaa gcttcgagac ctggaggact cactggcccg tgagcgggac accagccggc
     1801 ggctgctggc ggaaaaggag cgggagatgg ccgagatgcg ggcaaggatg cagcagcagc
     1861 tggacgagta ccaggagctt ctggacatca agctggccct ggacatggag atccacgcct
     1921 accgcaagct cttggagggc gaggaggaga ggctacgcct gtcccccagc cctacctcgc
     1981 agcgcagccg tggccgtgct tcctctcact catcccagac acagggtggg ggcagcgtca
     2041 ccaaaaagcg caaactggag tccactgaga gccgcagcag cttctcacag cacgcacgca
     2101 ctagcgggcg cgtggccgtg gaggaggtgg atgaggaggg caagtttgtc cggctgcgca
     2161 acaagtccaa tgaggaccag tccatgggca attggcagat caagcgccag aatggagatg
     2221 atcccttgct gacttaccgg ttcccaccaa agttcaccct gaaggctggg caggtggtga
     2281 cgatctgggc tgcaggagct ggggccaccc acagcccccc taccgacctg gtgtggaagg
     2341 cacagaacac ctggggctgc gggaacagcc tgcgtacggc tctcatcaac tccactgggg
     2401 aagaagtggc catgcgcaag ctggtgcgct cagtgactgt ggttgaggac gacgaggatg
     2461 aggatggaga tgacctgctc catcaccacc acggctccca ctgcagcagc tcgggggacc
     2521 ccgctgagta caacctgcgc tcgcgcaccg tgctgtgcgg gacctgcggg cagcctgccg
     2581 acaaggcatc tgccagcggc tcaggagccc aggtgggcgg acccatctcc tctggctctt
     2641 ctgcctccag tgtcacggtc actcgcagct accgcagtgt ggggggcagt gggggtggca
     2701 gcttcgggga caatctggtc acccgctcct acctcctggg caactccagc ccccgaaccc
     2761 agagccccca gaactgcagc atcatgtaag gatccaccgg atctagat
//
