LOCUS       sequence-84305-e        2825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2825)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-LaminA-N-18
FEATURES             Location/Qualifiers
     source          1..2825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2068..2077
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2074..2076,2077..2079,2080..2793)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2119..2140)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2380..2399)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2727..2748
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agtgacgtca gatccgctag cgccaccatg gagaccccgt cccagcggcg cgccacccgc
       61 agcggggcgc aggccagctc cactccgctg tcgcccaccc gcatcacccg gctgcaggag
      121 aaggaggacc tgcaggagct caatgatcgc ttggcggtct acatcgaccg tgtgcgctcg
      181 ctggaaacgg agaacgcagg gctgcgcctt cgcatcaccg agtctgaaga ggtggtcagc
      241 cgcgaggtgt ccggcatcaa ggccgcctac gaggccgagc tcggggatgc ccgcaagacc
      301 cttgactcag tagccaagga gcgcgcccgc ctgcagctgg agctgagcaa agtgcgtgag
      361 gagtttaagg agctgaaagc gcgcaatacc aagaaggagg gtgacctgat agctgctcag
      421 gctcggctga aggacctgga ggctctgctg aactccaagg aggccgcact gagcactgct
      481 ctcagtgaga agcgcacgct ggagggcgag ctgcatgatc tgcggggcca ggtggccaag
      541 cttgaggcag ccctaggtga ggccaagaag caacttcagg atgagatgct gcggcgggtg
      601 gatgctgaga acaggctgca gaccatgaag gaggaactgg acttccagaa gaacatctac
      661 agtgaggagc tgcgtgagac caagcgccgt catgagaccc gactggtgga gattgacaat
      721 gggaagcagc gtgagtttga gagccggctg gcggatgcgc tgcaggaact gcgggcccag
      781 catgaggacc aggtggagca gtataagaag gagctggaga agacttattc tgccaagctg
      841 gacaatgcca ggcagtctgc tgagaggaac agcaacctgg tgggggctgc ccacgaggag
      901 ctgcagcagt cgcgcatccg catcgacagc ctctctgccc agctcagcca gctccagaag
      961 cagctggcag ccaaggaggc gaagcttcga gacctggagg actcactggc ccgtgagcgg
     1021 gacaccagcc ggcggctgct ggcggaaaag gagcgggaga tggccgagat gcgggcaagg
     1081 atgcagcagc agctggacga gtaccaggag cttctggaca tcaagctggc cctggacatg
     1141 gagatccacg cctaccgcaa gctcttggag ggcgaggagg agaggctacg cctgtccccc
     1201 agccctacct cgcagcgcag ccgtggccgt gcttcctctc actcatccca gacacagggt
     1261 gggggcagcg tcaccaaaaa gcgcaaactg gagtccactg agagccgcag cagcttctca
     1321 cagcacgcac gcactagcgg gcgcgtggcc gtggaggagg tggatgagga gggcaagttt
     1381 gtccggctgc gcaacaagtc caatgaggac cagtccatgg gcaattggca gatcaagcgc
     1441 cagaatggag atgatccctt gctgacttac cggttcccac caaagttcac cctgaaggct
     1501 gggcaggtgg tgacgatctg ggctgcagga gctggggcca cccacagccc ccctaccgac
     1561 ctggtgtgga aggcacagaa cacctggggc tgcgggaaca gcctgcgtac ggctctcatc
     1621 aactccactg gggaagaagt ggccatgcgc aagctggtgc gctcagtgac tgtggttgag
     1681 gacgacgagg atgaggatgg agatgacctg ctccatcacc accacggctc ccactgcagc
     1741 agctcggggg accccgctga gtacaacctg cgctcgcgca ccgtgctgtg cgggacctgc
     1801 gggcagcctg ccgacaaggc atctgccagc ggctcaggag cccaggtggg cggacccatc
     1861 tcctctggct cttctgcctc cagtgtcacg gtcactcgca gctaccgcag tgtggggggc
     1921 agtgggggtg gcagcttcgg ggacaatctg gtcacccgct cctacctcct gggcaactcc
     1981 agcccccgaa cccagagccc ccagaactgc agcatcatga gcagcggtgg agcaagcgca
     2041 gccagtggta gcgcggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc
     2101 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     2161 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2221 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttgac ctacggcgtg
     2281 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     2341 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     2401 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     2461 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     2521 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc
     2581 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     2641 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
     2701 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     2761 atcactctcg gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag
     2821 ccata
//
