LOCUS       sequence-84306-e        2556 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Day RN, Davidson MW
  TITLE     The fluorescent protein palette: tools for cellular imaging.
  JOURNAL   Chem Soc Rev. 2009 Oct;38(10):2887-921. doi: 10.1039/b901966a. Epub 
            2009 Aug 4.
  PUBMED    19771335
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1039/b901966a"; 
            journalName: "Chem Soc Rev"; date: "2009-10"; volume: "38"; issue: 
            "10"; pages: "2887-921"
COMMENT     Sequence Label: mEmerald-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..2556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(32..34,35..37,38..748)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(77..98)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(338..357)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     685..706
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac
       61 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      121 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      181 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttgacct acggcgtgca
      241 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      301 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      361 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      421 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
      481 ggtctatatc accgccgaca agcagaagaa cggcatcaag gtgaacttca agacccgcca
      541 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      601 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
      661 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
      721 cactctcggc atggacgagc tgtacaagtc cggactcaga tctcgagctc aagcttcgat
      781 ggcgactgcg acccccgtgc cgccgcggat gggcagccgc gctggcggcc ccaccacgcc
      841 gctgagcccc acgcgcctgt cgcggctcca ggagaaggag gagctgcgcg agctcaatga
      901 ccggctggcg gtgtacatcg acaaggtgcg cagcctggag acggagaaca gcgcgctgca
      961 gctgcaggtg acggagcgcg aggaggtgcg cggccgtgag ctcaccggcc tcaaggcgct
     1021 ctacgagacc gagctggccg acgcgcgacg cgcgctcgac gacacggccc gcgagcgcgc
     1081 caagctgcag atcgagctgg gcaagtgcaa ggcggaacac gaccagctgc tcctcaacta
     1141 tgctaagaag gaatctgatc ttaatggcgc ccagatcaag cttcgagaat atgaagcagc
     1201 actcaattcg aaagatgcag ctcttgctac tgcacttggt gacaaaaaaa gtttagaggg
     1261 agatttggag gatctgaagg atcagattgc ccagttggaa gcctccttag ctgcagccaa
     1321 aaaacagtta gcagatgaaa ctttacttaa agtagatttg gagaatcgtt gtcagagcct
     1381 tactgaggac ttggagtttc gcaaaagcat gtatgaagag gagattaacg agaccagaag
     1441 gaagcatgaa acgcgcttgg tagaggtgga ttctgggcgt caaattgagt atgagtacaa
     1501 gctggcgcaa gcccttcatg agatgagaga gcaacatgat gcccaagtga ggctgtataa
     1561 ggaggagctg gagcagactt accatgccaa acttgagaat gccagactgt catcagagat
     1621 gaatacttct actgtcaaca gtgccaggga agaactgatg gaaagccgca tgagaattga
     1681 gagcctttca tcccagcttt ctaatctaca gaaagagtct agagcatgtt tggaaaggat
     1741 tcaagaatta gaggacttgc ttgctaaaga aaaagacaac tctcgtcgca tgctgacaga
     1801 caaagagaga gagatggcgg aaataaggga tcaaatgcag caacagctga atgactatga
     1861 acagcttctt gatgtaaagt tagccctgga catggaaatc agtgcttaca ggaaactctt
     1921 agaaggcgaa gaagagaggt tgaagctgtc tccaagccct tcttcccgtg tgacagtatc
     1981 ccgagcatcc tcaagtcgta gtgtacgtac aactagagga aagcggaaga gggttgatgt
     2041 ggaagaatca gaggcgagta gtagtgttag catctctcat tccgcctcag ccactggaaa
     2101 tgtttgcatc gaagaaattg atgttgatgg gaaatttatc cgcttgaaga acacttctga
     2161 acaggatcaa ccaatgggag gctgggagat gatcagaaaa attggagaca catcagtcag
     2221 ttataaatat acctcaagat atgtgctgaa ggcaggccag actgttacaa tttgggctgc
     2281 aaacgctggt gtcacagcca gccccccaac tgacctcatc tggaagaacc agaactcgtg
     2341 gggcactggc gaagatgtga aggttatatt gaaaaattct cagggagagg aggttgctca
     2401 aagaagtaca gtctttaaaa caaccatacc tgaagaagag gaggaggagg aagaagcagc
     2461 tggagtggtt gttgaggaag aacttttcca ccagcaggga accccaagag catccaatag
     2521 aagctgtgca attatgtagg gatccaccgg atctag
//
