LOCUS       sequence-84307-e        1609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1609)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-LASP1-C-10
FEATURES             Location/Qualifiers
     source          1..1609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(33..35,36..38,39..749)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(78..99)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(339..358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     686..707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      964..973
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca
       61 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      121 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      181 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttgacc tacggcgtgc
      241 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      301 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      361 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      421 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      481 aggtctatat caccgccgac aagcagaaga acggcatcaa ggtgaacttc aagacccgcc
      541 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      601 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
      661 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      721 tcactctcgg catggacgag ctgtacaagt ccggactcag atctagcggt ggcagtggaa
      781 accccaactg cgcccggtgc ggcaagatcg tgtatcccac ggagaaggtg aactgtctgg
      841 ataagttctg gcataaagca tgcttccatt gcgagacctg caagatgaca ctgaacatga
      901 agaactacaa gggctacgag aagaagccct actgcaacgc acactacccc aagcagtcct
      961 tcaccatggt ggcggacacc ccggaaaacc ttcgcctcaa gcaacagagt gagctccaga
     1021 gtcaggtgcg ctacaaggag gagtttgaga agaacaaggg caaaggtttc agcgtagtgg
     1081 cagacacgcc cgagctccag agaatcaaga agacccagga ccagatcagt aacataaaat
     1141 accatgagga gtttgagaag agccgcatgg gccctagcgg gggcgagggc atggagccag
     1201 agcgtcggga ttcacaggac ggcagcagct accggcggcc cctggagcag cagcagcctc
     1261 accacatccc gaccagtgcc ccggtttacc agcagcccca gcagcagccg gtggcccagt
     1321 cctatggtgg ctacaaggag cctgcagccc cagtctccat acagcgcagc gccccaggtg
     1381 gtggcgggaa gcggtaccgc gtggtgtatg actacagcgc cgccgacgag gacgaggtct
     1441 ccttccagga cggggacacc atcgtcaacg tgcagcagat cgacgacggc tggatgtacg
     1501 ggacggtgga gcgcaccggc gacacgggga tgctgccggc caactacgtg gaggccatct
     1561 gaggatccac cggatctaga taactgatca taatcagcca taccacatt
//
