LOCUS       sequence-84308-e        1597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1597)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-LASP1-N-10
FEATURES             Location/Qualifiers
     source          1..1597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      214..223
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      834..843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(840..842,843..845,846..1559)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(885..906)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1146..1165)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1493..1514
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtgacgtcag atccgctagc gccaccatga accccaactg cgcccggtgc ggcaagatcg
       61 tgtatcccac ggagaaggtg aactgtctgg ataagttctg gcataaagca tgcttccatt
      121 gcgagacctg caagatgaca ctgaacatga agaactacaa gggctacgag aagaagccct
      181 actgcaacgc acactacccc aagcagtcct tcaccatggt ggcggacacc ccggaaaacc
      241 ttcgcctcaa gcaacagagt gagctccaga gtcaggtgcg ctacaaggag gagtttgaga
      301 agaacaaggg caaaggtttc agcgtagtgg cagacacgcc cgagctccag agaatcaaga
      361 agacccagga ccagatcagt aacataaaat accatgagga gtttgagaag agccgcatgg
      421 gccctagcgg gggcgagggc atggagccag agcgtcggga ttcacaggac ggcagcagct
      481 accggcggcc cctggagcag cagcagcctc accacatccc gaccagtgcc ccggtttacc
      541 agcagcccca gcagcagccg gtggcccagt cctatggtgg ctacaaggag cctgcagccc
      601 cagtctccat acagcgcagc gccccaggtg gtggcgggaa gcggtaccgc gtggtgtatg
      661 actacagcgc cgccgacgag gacgaggtct ccttccagga cggggacacc atcgtcaacg
      721 tgcagcagat cgacgacggc tggatgtacg ggacggtgga gcgcaccggc gacacgggga
      781 tgctgccggc caactacgtg gaggccatca gctccggagg ggatccaccg gtcgccacca
      841 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      901 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      961 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     1021 tcgtgaccac cttgacctac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc
     1081 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     1141 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     1201 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     1261 agctggagta caactacaac agccacaagg tctatatcac cgccgacaag cagaagaacg
     1321 gcatcaaggt gaacttcaag acccgccaca acatcgagga cggcagcgtg cagctcgccg
     1381 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     1441 acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     1501 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag
     1561 cggccgcgac tctagatcat aatcagccat accacat
//
