LOCUS       sequence-84309-e        1673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1673)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-LASP2-C-18
FEATURES             Location/Qualifiers
     source          1..1673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(32..34,35..37,38..748)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(77..98)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(338..357)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     685..706
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac
       61 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      121 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      181 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttgacct acggcgtgca
      241 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      301 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      361 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      421 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
      481 ggtctatatc accgccgaca agcagaagaa cggcatcaag gtgaacttca agacccgcca
      541 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      601 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
      661 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
      721 cactctcggc atggacgagc tgtacaagtc cggactcaga tctcgagagt ccagtggata
      781 ttcgtctcgg tcgggtggaa gcaaccccca gtgcgcccgc tgtgggaaag tggtctatcc
      841 caccgagaaa gtcaactgcc tggacaagta ttggcataaa ggatgtttcc actgtgaagt
      901 ttgcaaaatg gctctgaaca tgaataacta caaaggatat gaaaagaaac cttattgcaa
      961 tgcacattac ccaaagcagt ccttcactac agtggctgat actccagaaa atctacgtct
     1021 gaagcaacaa agtgaattgc agagtcaggt aaaatacaaa agagactttg aagaaagcaa
     1081 aggaagaggg ttcagtattg tgaccgatac gcccgagtta caaagactga aaaggactca
     1141 ggaacaaatc agtaatgtta aataccatga agattttgag aagacaaagg gcaggggttt
     1201 cacgccagtg gtggatgatc ctataacaga gcgggtgcgg aaaaacaccc aagttgtaag
     1261 tgatgcagcg tataaagggg tgcacccaca tatagttgaa atggatagaa gacctggaat
     1321 aattgttgcc cctgtactcc ctggggcata tcagcaaagc cagtcccaag gatatggata
     1381 catgcaccag accagtatgt catctatgag gtcagtgcat tcacagcctc atccagcagg
     1441 cctgagaaca tacagagcta tgtatgacta cagtgctcaa gatgaagatg aagtttcctt
     1501 cagggatggt gactatatca tcaacgtgca accaattgat gatggctgga tgtatggtac
     1561 tgttcagaga actgggaaaa caggaatgct tccagcaaat tatattgagt ttgttaacta
     1621 agaattctgc agtcgacggt accgcgggcc cgggatccac cggatctaga taa
//
