LOCUS       sequence-84310-e        1650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1650)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-LASP2-N-18
FEATURES             Location/Qualifiers
     source          1..1650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      893..902
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(899..901,902..904,905..1618)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(944..965)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1205..1224)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1552..1573
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ccaccatgaa cccccagtgc gcccgctgtg ggaaagtggt
       61 ctatcccacc gagaaagtca actgcctgga caagtattgg cataaaggat gtttccactg
      121 tgaagtttgc aaaatggctc tgaacatgaa taactacaaa ggatatgaaa agaaacctta
      181 ttgcaatgca cattacccaa agcagtcctt cactacagtg gctgatactc cagaaaatct
      241 acgtctgaag caacaaagtg aattgcagag tcaggtaaaa tacaaaagag actttgaaga
      301 aagcaaagga agagggttca gtattgtgac cgatacgccc gagttacaaa gactgaaaag
      361 gactcaggaa caaatcagta atgttaaata ccatgaagat tttgagaaga caaagggcag
      421 gggtttcacg ccagtggtgg atgatcctat aacagagcgg gtgcggaaaa acacccaagt
      481 tgtaagtgat gcagcgtata aaggggtgca cccacatata gttgaaatgg atagaagacc
      541 tggaataatt gttgcccctg tactccctgg ggcatatcag caaagccagt cccaaggata
      601 tggatacatg caccagacca gtatgtcatc tatgaggtca gtgcattcac agcctcatcc
      661 agcaggcctg agaacataca gagctatgta tgactacagt gctcaagatg aagatgaagt
      721 ttccttcagg gatggtgact atatcatcaa cgtgcaacca attgatgatg gctggatgta
      781 tggtactgtt cagagaactg ggaaaacagg aatgcttcca gcaaattata ttgagtttgt
      841 taacagtcgc ggaggcagtg gtggggtacc gcgggcccgg gatccaccgg tcgccaccat
      901 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      961 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1021 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1081 cgtgaccacc ttgacctacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
     1141 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1201 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1261 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1321 gctggagtac aactacaaca gccacaaggt ctatatcacc gccgacaagc agaagaacgg
     1381 catcaaggtg aacttcaaga cccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1441 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1501 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1561 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc
     1621 ggccgcgact ctagatcata atcagccata
//
