LOCUS       sequence-84312-e        1339 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Light Chain Myosin, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1339)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-LC-Myosin-N-7
FEATURES             Location/Qualifiers
     source          1..1339
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      575..584
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(581..583,584..586,587..1300)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(626..647)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(887..906)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1234..1255
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agatccgcta gcgctaccgg actcagatct cgagctcaag cttatgtcga gcaagagagc
       61 caaggccaag accaccaaga agaggcccca gagggctacg tccaatgtct tcgccatgtt
      121 tgaccagtcc cagatccagg agtttaagga ggccttcaac atgattgatc agaaccgcga
      181 tggcttcatt gataaggagg acctgcacga catgttggcc tctctgggga agaaccccac
      241 agacgagtac ctggagggca tgatgaacga ggcgccaggg cccatcaact tcaccatgtt
      301 cctcacaatg tttggggaga agctgaacgg cacagacccc gaggatgtga tccgcaatgc
      361 ctttgcctgc tttgatgagg aggcctcagg cttcatccac gaggaccacc tgagggagct
      421 gctcaccacc atgggcgacc gattcacgga tgaggaggtg gacgagatgt accgcgaggc
      481 acccattgat aagaagggca acttcaacta cgtggagttc actcgcatcc tcaaacacgg
      541 cgccaaggac aaggacgacc tggatccacc ggtcgccacc atggtgagca agggcgagga
      601 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      661 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      721 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttgaccta
      781 cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      841 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
      901 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
      961 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     1021 cagccacaag gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     1081 gacccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     1141 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa
     1201 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1261 cgccgggatc actctcggca tggacgagct gtacaagtaa gcggccgcga ctctagatca
     1321 taatcagcca taccacatt
//
