LOCUS       sequence-84315-e        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Planchon TA, Gao L, Milkie DE, Davidson MW, Galbraith JA, Galbraith 
            CG, Betzig E
  TITLE     Rapid three-dimensional isotropic imaging of living cells using 
            Bessel beam plane illumination.
  JOURNAL   Nat Methods. 2011 May;8(5):417-23. doi: 10.1038/nmeth.1586. Epub 
            2011 Mar 4.
  PUBMED    21378978
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1586"; journalName: "Nat Methods"; date: "2011-05"; 
            volume: "8"; issue: "5"; pages: "417-23"
COMMENT     Sequence Label: Emerald-MannII-N-10
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      385..394
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(391..393,394..396,397..1110)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(436..457)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(697..716)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1044..1065
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acggtcagat ccgctagcgc caccatgaag ttaagtcgcc agttcaccgt gtttggcagc
       61 gcgatcttct gcgtcgtaat cttctcactc tacctgatgc tggacagggg tcacttggac
      121 taccctcggg gcccgcgcca ggagggctcc tttccgcagg gccagctttc aatattgcaa
      181 gaaaagattg accatttgga gcgtttgctc gctgagaaca acgagatcat ctcaaatatc
      241 agagactcag tcatcaacct gagcgagtct gtggaggacg gcccgcgggg gtcaccaggc
      301 aacgccagcc aaggctccat ccacctccac tcgccacagt tggccctgca ggctgacccc
      361 agctccggat cggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
      421 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      481 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      541 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttgaccta cggcgtgcag
      601 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      661 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      721 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      781 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaag
      841 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa gacccgccac
      901 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
      961 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa
     1021 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1081 actctcggca tggacgagct gtacaagtaa gcggccgcga ctctagatca taatca
//
