LOCUS       sequence-84319-e        1890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1890)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-MAPTau-N-10
FEATURES             Location/Qualifiers
     source          1..1890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1126..1135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1132..1134,1135..1137,1138..1851)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1177..1198)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1438..1457)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1785..1806
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgttcagatc cgctagcgct accggactca gatctcgagg ccaccatggc tgagccccgc
       61 caggagttcg aagtgatgga agatcacgct gggacgtacg ggttggggga caggaaagat
      121 caggggggct acaccatgca ccaagaccaa gagggtgaca cggacgctgg cctgaaagct
      181 gaagaagcag gcattggaga cacccccagc ctggaagacg aagctgctgg tcacgtgacc
      241 caagctcgca tggtcagtaa aagcaaagac gggactggaa gcgatgacaa aaaagccaag
      301 ggggctgatg gtaaaacgaa gatcgccaca ccgcggggag cagcccctcc aggccagaag
      361 ggccaggcca acgccaccag gattccagca aaaaccccgc ccgctccaaa gacaccaccc
      421 agctctggtg aacctccaaa atcaggggat cgcagcggct acagcagccc cggctcccca
      481 ggcactcccg gcagccgctc ccgcaccccg tcccttccaa ccccacccac ccgggagccc
      541 aagaaggtgg cagtggtccg tactccaccc aagtcgccgt cttccgccaa gagccgcctg
      601 cagacagccc ccgtgcccat gccagacctg aagaatgtca agtccaagat cggctccact
      661 gagaacctga agcaccagcc gggaggcggg aaggtgcaaa tagtctacaa accagttgac
      721 ctgagcaagg tgacctccaa gtgtggctca ttaggcaaca tccatcataa accaggaggt
      781 ggccaggtgg aagtaaaatc tgagaagctt gacttcaagg acagagtcca gtcgaagatt
      841 gggtccctgg acaatatcac ccacgtccct ggcggaggaa ataaaaagat tgaaacccac
      901 aagctgacct tccgcgagaa cgccaaagcc aagacagacc acggggcgga gatcgtgtac
      961 aagtcgccag tggtgtctgg ggacacgtct ccacggcatc tcagcaatgt ctcctccacc
     1021 ggcagcatcg acatggtaga ctcgccccag ctcgccacgc tagctgacga ggtgtctgcc
     1081 tccctggcca agcagggttt gagctccgga ggggatccac cggtcgccac catggtgagc
     1141 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1201 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     1261 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     1321 accttgacct acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     1381 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     1441 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1501 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1561 tacaactaca acagccacaa ggtctatatc accgccgaca agcagaagaa cggcatcaag
     1621 gtgaacttca agacccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     1681 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     1741 acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     1801 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta agcggccgcg
     1861 actctagatc ataatcagcc ataccacatt
//
