LOCUS       sequence-84321-e        4635 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4635)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-mDia1-N-14
FEATURES             Location/Qualifiers
     source          1..4635
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      3859..3868
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3865..3867,3868..3870,3871..4584)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3910..3931)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4171..4190)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4518..4539
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 accatgctag tgacgtcaga tccgctagcg ctaccggact cagatctcga gaccgccatg
       61 gagccgtccg gcgggggcct ggggcccggc cgcggtaccc gggacaagaa gaagggtcgg
      121 agcccggatg agctgcctgc gacgggcggc gacggcggca aacataagaa atttctggag
      181 agatttacca gcatgaggat taagaaggag aaagaaaagc ccaattctgc tcatagaaac
      241 tcctctgcat cgtacggaga tgaccccact gctcagtcat tgcaggacat ctcagacgag
      301 caagttcttg tcctctttga gcagatgctg gtggatatga acctgaatga ggagaagcag
      361 cagcctttgc gagagaagga cattgtcatc aagagggaga tggtgtcgca atatctgcac
      421 acttccaagg ctggcatgaa ccagaaagag agctctaggt ctgccatgat gtacatccag
      481 gagctgaggt cgggcttgcg ggatatgcac ctgcttagct gccttgagtc ccttcgagtc
      541 tctctcaaca ataaccctgt cagttgggtg cagacatttg gtgctgaggg cctagcctcc
      601 ttattggaca tcctcaaacg actccatgat gagaaagagg agacttctgg aaactacgac
      661 agccgaaacc agcatgagat tatccgctgt ttgaaggctt tcatgaacaa caagtttgga
      721 atcaaaacta tgttggagac ggaagaagga atcctactgc tggtcagagc catggaccct
      781 gctgttccca atatgatgat tgatgcagca aagctgctgt ctgccctctg tatcctgccg
      841 cagccggagg acatgaatga acgagttcta gaggcaatga cagagagagc tgaaatggat
      901 gaggtcgaac gcttccagcc acttctggac ggattaaaaa gtgggacctc tattgccctc
      961 aaagtgggat gcctacagct catcaatgct ctcatcactc cagctgagga actggacttc
     1021 cgagttcaca tccgaagtga gctgatgcgc ctggggctgc atcaggtgtt gcaggagctt
     1081 cgagagattg aaaatgaaga tatgaaagta cagctgtgcg tgtttgatga acaaggggat
     1141 gaagatttct ttgatctgaa gggacggctg gatgatatcc gcatggagat ggatgacttt
     1201 ggtgaagttt ttcagattat tttaaacaca gtgaaagatt caaaggcaga gccacacttc
     1261 ctgtctatct tgcagcatct cctgttggtc cgaaatgatt atgaagccag gccacagtac
     1321 tataaactga ttgaagaatg tgtttctcag atagttctac acaaaaatgg aactgatcct
     1381 gacttcaagt gccgacacct gcagattgat attgagagat tggttgatca aatgattgat
     1441 aaaacaaagg tggaaaaatc tgaggccaaa gctacagagc tggaaaaaaa gttggattca
     1501 gaattaacag cgcggcacga gttacaagta gaaatgaaaa agatggaaaa tgactttgag
     1561 cagaaacttc aggatcttca aggagaaaag gatgccttgg attctgaaaa gcagcagatc
     1621 actgcacaga aacaagacct ggaggcagag gtgtccaagc tgacaggaga ggttgccaag
     1681 ctgtcaaaag aactagaaga tgccaagaat gaaatggctt ctctctctgc tgtggttgtt
     1741 gcaccttctg tttctagcag tgctgctgtt ccccctgccc ctcctctgcc tggtgactct
     1801 ggcactgtta ttccacctcc cccaccccca cctcctcttc ctggaggtgt ggtcccacca
     1861 tcccctcctc tgcctccagg tacttgtatc cctccacctc ctcctttacc tggaggtgct
     1921 tgtatacccc ctccccccca gttgcctggc agtgctgcca tccctccacc tcctcctcta
     1981 cctggagttg cttccatccc cccacctccc cctttgcctg gggctactgc catcccccca
     2041 cctccccctt tgcctggggc tactgccatc cccccacctc cccctttgcc tggaggtaca
     2101 ggtataccac caccacctcc tcctttgcct ggaagtgttg gcgttccccc accccctccc
     2161 ttgcctggag gaccaggact gcctcctccc cctccccctt ttcctggagc acctggcatt
     2221 cctccacctc cacctggtat gggcgtgcct ccacctcccc cctttggatt tggggttcct
     2281 gcggccccag ttctgccatt tggattaacc cccaaaaaag tttataagcc agaggtgcag
     2341 ctccgcaggc caaactggtc caagtttgtg gctgaggacc tttcccagga ctgcttctgg
     2401 acaaaggtga aggaggaccg ctttgagaac aatgaacttt ttgccaaact tacccttgcc
     2461 ttctccgccc agaccaagac ttctaaagcc aagaaggatc aagaaggtgg agaagaaaag
     2521 aaatctgttc aaaagaagaa agtaaaagag ctgaaagtgc tggattcaaa gacagcgcag
     2581 aatctctcaa tctttttggg ttcattccgc atgccctatc aagagataaa gaacgttatc
     2641 ctggaggtga atgaggctgt tctcacagag tctatgatcc agaacctcat taaacagatg
     2701 ccagagccag agcagctaaa gatgctctct gaactgaagg aggagtacga tgatctggct
     2761 gagtcagagc agtttggtgt ggtgatgggc acagtgcccc gccttcggcc tcgcctcaac
     2821 gccatcctct tcaagctaca gttcagtgag caagttgaga acatcaagcc agagatcgtg
     2881 tctgtcaccg ccgcatgcga agagctgcgt aagagtgaga acttctccag cctcctggag
     2941 ctcacactgc tggtcggaaa ctatatgaat gcgggctcca ggaatgctgg tgctttcggc
     3001 ttcaatatca gcttcctttg taaacttcga gacaccaagt ctgcagatca gaagatgact
     3061 ctgttgcatt tcttggctga gttatgtgag aatgaccacc ccgaagtcct caagtttcct
     3121 gatgagcttg cccatgtaga gaaagccagc agagtctctg ctgagaacct gcagaagagc
     3181 ttagatcaga tgaagaagca gattgcggac gtggagcgcg atgttcagaa tttcccagct
     3241 gccactgacg agaaggacaa gtttgttgag aagatgacca gctttgtgaa ggatgcacag
     3301 gaacagtata acaaactacg gatgatgcac tccaacatgg agaccctcta taaggagcta
     3361 ggtgactact tcgtctttga ccctaagaag ttgtctgtag aggagttctt tatggatctg
     3421 cacaacttta ggaatatgtt tttgcaagca gtcaaggaaa accagaagcg ccgggaaaca
     3481 gaagaaaaga tgcggagagc aaaattagcc aaggagaagg cagaaaaaga gcgactggag
     3541 aagcagcaga agcgcgagca gctcatcgac atgaacgcag agggggatga gacaggtgtg
     3601 atggacagtc ttctagaagc tctgcagtca ggggcagcat tccgacggaa gagagggccc
     3661 cggcaggtca acaggaaggc tgggtgtgca gtcacatctc tgctggcctc ggagctgacc
     3721 aaggatgatg ccatggctcc tggtcctgtt aaggtaccca agaaaagtga aggagtcccc
     3781 acaatcctgg aagaagccaa ggagctggtt ggccgtgcaa gcgggggtgc gtcaggcggc
     3841 agcggatctg gaccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     3901 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3961 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     4021 aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt gcagtgcttc
     4081 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     4141 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     4201 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     4261 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caaggtctat
     4321 atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg ccacaacatc
     4381 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     4441 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc
     4501 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     4561 ggcatggacg agctgtacaa gtaagcggcc gcgactctag atcataatca gccataccac
     4621 attgtagaag tatcc
//
