LOCUS       sequence-84325-e        3037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules/Kinesin, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3037)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-MCAK-C-7
FEATURES             Location/Qualifiers
     source          1..3037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     681..702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2944..2996
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc
      241 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagtccgga gccgcttccg cctccatggc catggactcg
      781 tcgcttcagg cccgcctgtt tcccggtctc gctatcaaga tccaacgcag taatggttta
      841 attcacagtg ccaatgtaag gactgtgaac ttggagaaat cctgtgtttc agtggaatgg
      901 gcagaaggag gtgccacaaa gggcaaagag attgattttg atgatgtggc tgcaataaac
      961 ccagaactct tacagcttct tcccttacat ccgaaggaca atctgccctt gcaggaaaat
     1021 gtaacaatcc agaaacaaaa acggagatcc gtcaactcca aaattcctgc tccaaaagaa
     1081 agtcttcgaa gccgctccac tcgcatgtcc actgtctcag agcttcgcat cacggctcag
     1141 gagaatgaca tggaggtgga gctgcctgca gctgcaaact cccgcaagca gttttcagtt
     1201 cctcctgccc ccactaggcc ttcctgccct gcagtggctg aaataccatt gaggatggtc
     1261 agcgaggaga tggaagagca agtccattcc atccgaggca gctcttctgc aaaccctgtg
     1321 aactcagttc ggaggaaatc atgtcttgtg aaggaagtgg aaaaaatgaa gaacaagcga
     1381 gaagagaaga aggcccagaa ctctgaaatg agaatgaaga gagctcagga gtatgacagt
     1441 agttttccaa actgggaatt tgcccgaatg attaaagaat ttcgggctac tttggaatgt
     1501 catccactta ctatgactga tcctatcgaa gagcacagaa tatgtgtctg tgttaggaaa
     1561 cgcccactga ataagcaaga attggccaag aaagaaattg atgtgatttc cattcctagc
     1621 aagtgtctcc tcttggtaca tgaacccaag ttgaaagtgg acttaacaaa gtatctggag
     1681 aaccaagcat tctgctttga ctttgcattt gatgaaacag cttcgaatga agttgtctac
     1741 aggttcacag caaggccact ggtacagaca atctttgaag gtggaaaagc aacttgtttt
     1801 gcatatggcc agacaggaag tggcaagaca catactatgg gcggagacct ctctgggaaa
     1861 gcccagaatg catccaaagg gatctatgcc atggcctccc gggacgtctt cctcctgaag
     1921 aatcaaccct gctaccggaa gttgggcctg gaagtctatg tgacattctt cgagatctac
     1981 aatgggaagc tgtttgacct gctcaacaag aaggccaagc tgcgcgtgct ggaggacggc
     2041 aagcaacagg tgcaagtggt ggggctgcag gagcatctgg ttaactctgc tgatgatgtc
     2101 atcaagatga tcgacatggg cagcgcctgc agaacctctg ggcagacatt tgccaactcc
     2161 aattcctccc gctcccacgc gtgcttccaa attattcttc gagctaaagg gagaatgcat
     2221 ggcaagttct ctttggtaga tctggcaggg aatgagcgag gcgcggacac ttccagtgct
     2281 gaccggcaga cccgcatgga gggcgcagaa atcaacaaga gtctcttagc cctgaaggag
     2341 tgcatcaggg ccctgggaca gaacaaggct cacaccccgt tccgtgagag caagctgaca
     2401 caggtgctga gggactcctt cattggggag aactctagga cttgcatgat tgccacgatc
     2461 tcaccaggca taagctcctg tgaatatact ttaaacaccc tgagatatgc agacagggtc
     2521 aaggagctga gcccccacag tgggcccagt ggagagcagt tgattcaaat ggaaacagaa
     2581 gagatggaag cctgctctaa cggggcgctg attccaggca atttatccaa ggaagaggag
     2641 gaactgtctt cccagatgtc cagctttaac gaagccatga ctcagatcag ggagctggag
     2701 gagaaggcta tggaagagct caaggagatc atacagcaag gaccagactg gcttgagctc
     2761 tctgagatga ccgagcagcc agactatgac ctggagacct ttgtgaacaa agcggaatct
     2821 gctctggccc agcaagccaa gcatttctca gccctgcgag atgtcatcaa ggccttgcgc
     2881 ctggccatgc agctggaaga gcaggctagc agacaaataa gcagcaagaa acggccccag
     2941 tgactcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg
     3001 gatctagata actgatcata atcagccata ccacatt
//
