LOCUS       sequence-84326-e        1867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1867)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-MCHR1-N-10
FEATURES             Location/Qualifiers
     source          1..1867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1102..1111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1108..1110,1111..1113,1114..1827)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1153..1174)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1414..1433)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1761..1782
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agatccgcta gcgccaccat ggatctgcaa gcctcgttgc tgtccactgg ccccaatgcc
       61 agcaacatct ccgatggcca ggataatttc acattggcgg ggccacctcc tcgcacaagg
      121 agtgtctcct acatcaacat catcatgcct tcagtgtttg gtaccatctg tctcctgggc
      181 attgtgggaa actccacagt catttttgcc gtggtgaaga aatccaagct gcactggtgc
      241 agcaacgtcc ctgacatctt catcatcaac ctctctgtgg tggatctgct tttcctgctg
      301 ggcatgcctt tcatgatcca ccagctcatg ggtaatggtg tctggcactt tggggaaacc
      361 atgtgcaccc tcatcacagc catggacgcc aacagtcagt tcaccagcac ctacatcctg
      421 actgctatgg ccattgaccg ctacttggcc accgtccatc ccatctcctc caccaagttc
      481 cggaagccct ccatggccac cctggtgatc tgcctcctgt gggctctctc gttcattagc
      541 atcactcctg tgtggctcta tgccaggctt atccccttcc cagggggtgc tgtgggctgt
      601 ggcatccgcc taccaaaccc agatactgat ctttactggt tcactctgta tcagtttttc
      661 ctggccttcg cccttccgtt tgtggtcatc actgctgcgt acgtgaaaat actacagcgc
      721 atgacgtctt cggtggcccc agcctctcaa cgcagcatcc ggcttcggac aaagagggtg
      781 acccgcacag ccattgccat ctgtctggtc ttctttgtgt gctgggcgcc ctactacgtg
      841 ctgcagctga cccagttgtc catcagccgc ccgaccctca cattcgtcta cctgtacaat
      901 gcggctatca gcttgggcta tgccaacagc tgcctcaatc cctttgtgta catagtactc
      961 tgtgagacct ttcgaaaacg cttggtgctg tcggtgaagc ccgcggccca ggggcagctt
     1021 cgcacggtca gcaatgctca gacagctgac gaggagagga cagaaagcaa aggcaccgga
     1081 ggtagcgggg atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
     1141 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1201 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1261 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc
     1321 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1381 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1441 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1501 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc
     1561 tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac
     1621 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1681 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
     1741 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1801 ctcggcatgg acgagctgta caagtaagcg gccgcgactc tagatcataa tcagccatac
     1861 cacattt
//
