LOCUS       sequence-84327-e        3252 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Kuipers MA, Stasevich TJ, Sasaki T, Wilson KA, Hazelwood KL, McNally
            JG, Davidson MW, Gilbert DM
  TITLE     Highly stable loading of Mcm proteins onto chromatin in living cells
            requires replication to unload.
  JOURNAL   J Cell Biol. 2011 Jan 10;192(1):29-41. doi: 10.1083/jcb.201007111.
  PUBMED    21220507
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2011-01-10- 10"; volume: "192"; 
            issue: "1"; pages: "29-41"
COMMENT     Sequence Label: mEmerald-MCM3-C-19
FEATURES             Location/Qualifiers
     source          1..3252
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttgac ctacggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      481 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc
      541 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggagaag ggcaagggca agggcaaggg
      781 ccgggccgcg gctacgcgta tcgatccatg gcgggcacag tagtgctgga tgatgtggag
      841 ctgcgggaag cgcagagaga ctacttggac ttcctggatg acgaggagga ccaaggcatt
      901 taccagaaca aggttcggga actgatcagt gacaaccagt atcggctgat tgtcagcgtg
      961 aatgacctgc gcaggaagaa tgaaaagagg gctaaccgcc tcctgaacaa tgcgtttgag
     1021 gagctggttg ccttccagcg ggccttgaag gatttcgtgg cctccattga cgccacctac
     1081 gccaagcagt atgaggagtt ctacatagga ttggaaggca gctttggctc aaagcacgtt
     1141 tctccccgga ctctcacttc ctgcttcctc agctgtgtgg tctgtgtgga aggcattgtt
     1201 actaaatgct ctctggttcg ccccaaagtc gttcgcagtg ttcactactg tcctgctact
     1261 aagaagacca tagagcgacg ctactctgac ctcaccaccc tagtggcttt cccatccagt
     1321 tctgtttatc ccacaaagga tgaggagaac aaccccctgg agacagagta tggcctgtct
     1381 gtctacaagg accaccagac catcaccatc caggagatgc cagagaaggc ccctgctggt
     1441 cagctccctc gctccgtgga cgtcattcta gacgatgacc tggtggataa agtgaagcct
     1501 ggtgacagaa tacaggtggt ggggacctac cgctgccttc cagggaagaa gggctgctac
     1561 acctcgggga ccttcaggac cgtcctgatt gcctgtaatg tgaagcagat gagtaaggat
     1621 attcagcctg cattctctgc ggacgatata gccaagatca agaagttcag caaaactcgt
     1681 tccaaggatg tctttgagca gctggcccgg tcgttagccc cgagcatcca tgggcatgac
     1741 tatgtcaaga aggcaatcct ctgcttgctc ctgggagggg tggagcggga acttgaaaac
     1801 ggcagccaca tccgtggaga catcaatatt cttctaatag gtgacccctc cgttgccaag
     1861 tcccagcttc tgcggtacgt gctttgcacg gcgcccaggg ccattcccac cacgggacgg
     1921 ggctcctctg gagtgggtct cacagctgct gtcactacag accaggaaac aggggagcgc
     1981 cgcctggaag ctggtgctat ggtcctggct gaccgtggag tggtttgcat tgatgagttt
     2041 gacaagatgt ctgacatgga ccgcacagcc atccacgagg tgatggagca gggtcgagtg
     2101 accattgcaa aggctggcat ccatgcccga ctcaatgccc gctgcagtgt tttggcagct
     2161 gccaatccag tctatggcag gtatgatcag tataagaccc ccatggagaa cattgggctg
     2221 caggactcac tgctgtctag atttgacctg ctcttcatca tgctggatca gatggaccct
     2281 gaacaggatc gggagatctc agaccacgtc cttaggatgc accaatacag ggccccgggg
     2341 gagcaggacg gtgatgcttt gccattgggt agttcagtgg atatcctggc cacagacgat
     2401 cctgacttta cccaggatga ccagcaagat acccgcattt atgagaagca tgacagcctt
     2461 ctacatggga ccaagaagaa aaaggagaag atggtgagcg cagcttttat gaagaagtac
     2521 attcacgtgg ccaaaattat caagcccacc ctgacacagg agtcagcagc ctacattgca
     2581 gaagaatact cacgcctgcg cagccaggac agtatgagct cagacactgc ccggacatct
     2641 ccagttacag cacggacact ggaaactctg attcgactgg ccacggccca tgcgaaggcc
     2701 cggatgagca agactgtgga cctgcaggat gcagaggaag ccgtggagct ggttcagtat
     2761 gcttatttca agaaggttct ggagaaggag aagaaacgta agaaggcaag tgaggatgaa
     2821 tcagacctag aagatgaaga ggaaaagagc caggaagaca ctgagcagaa gcggaaaagg
     2881 aggaagactc atgccaagga tggagagtcc tatgaccctt acgacttcag tgaagcagag
     2941 acgcagatgc ctcaagtgca caccccaaag actgacgatt cccaagagaa gaccgatgat
     3001 tctcaggaga cccaggactc ccagaaagtg gaactcagtg agcccaggct gaaggctttt
     3061 aaggcggccc tcttagaagt gttccaggaa gctcatgagc agtctgtggg catgctccac
     3121 ctcacagaat ccatcaaccg gaatagagaa gagcctttct cctcagagga aatccaggct
     3181 tgcctgagcc ggatgcagga tgataaccag gtcatggtgt ctgagggcat cgtcttcctt
     3241 atctgaggat cc
//
