LOCUS       sequence-84328-e        3408 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Kuipers MA, Stasevich TJ, Sasaki T, Wilson KA, Hazelwood KL, McNally
            JG, Davidson MW, Gilbert DM
  TITLE     Highly stable loading of Mcm proteins onto chromatin in living cells
            requires replication to unload.
  JOURNAL   J Cell Biol. 2011 Jan 10;192(1):29-41. doi: 10.1083/jcb.201007111.
  PUBMED    21220507
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2011-01-10- 10"; volume: "192"; 
            issue: "1"; pages: "29-41"
COMMENT     Sequence Label: mEmerald-MCM4-N-22
FEATURES             Location/Qualifiers
     source          1..3408
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2664..2673
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2670..2672,2673..2675,2676..3389)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2715..2736)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2976..2995)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3323..3344
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagatccgct agccaccatg tcgtccccgg catccacccc gagccgccgc agcagccgac
       61 gcggacgagt caccccaacc cagtcccttc gaagtgagga aagcaggtcg tcacccaatc
      121 ggagacgtag aggcgaagat tcttccaccg gagagctact gccaatgccc acctcaccag
      181 gagccgacct gcagagccca cctgcacaga atgccttgtt ttccagccct cctcagatgc
      241 attctttagc tattcctttg gactttgatg ttagttcacc attgacatat ggcactccca
      301 gctcgcgagt ggaaggaacc ccaagaagtg gggtgagagg cacacctgta aggcagaggc
      361 cagatctggg ctcagcacga aagggtttgc aggtggatct gcagtctgat ggcgcagcag
      421 cagaagacat cgtaccaagt gaacagtctc taggccaaaa gcttgtgatt tggggaacag
      481 atgtgaatgt ggcaacatgt aaagagaatt ttcagagatt ccttcagtgt tttactgatc
      541 ctctggccaa agaagaagaa aatgttggca tagatattac tcaacctttg tacatgcaac
      601 aacttggaga gattaatatt acaggagagc catttttaaa tgtgaactgc gaacacataa
      661 aatcatttag caaaaatctg tatagacagc tcatctccta cccacaggag gttataccaa
      721 cctttgacat ggctgtcaat gagatcttct ttgaccgtta tcctgactcc atcttagaac
      781 atcagattca agtcagacct tttaatgcgt tgaagacaaa gagtatgaga aacttgaatc
      841 cagaagacat tgatcagctc atcaccatca gtggcatggt catcagaaca tcacagctga
      901 ttccggagat gcaggaggcc tttttccaat gccaagtctg tgcccacacc acccgggtgg
      961 agatagatcg aggcagaatt gctgagccct gcagttgtgt gcactgccac actacccaca
     1021 gcatggcact gatccacaac cgatcattct tctctgacaa gcaaatgatc aaacttcaag
     1081 agtctcctga agacatgcct gctgggcaga cacctcacac tattgtcctt tttgcccaca
     1141 atgaccttgt tgacaaggtt caaccagggg acagagtgaa cgtcacaggc atatatcgag
     1201 cagtaccaat tcgagttaat ccaagagtga gcaacgtgaa gtctgtctat aaaacccaca
     1261 ttgatgtcat tcattatcgg aaaacggatg caaaacgtct gcatggcctt gatgaagaag
     1321 cagaacagaa acttttttca gagaaacgtg tgaaattgct taaggaactt tccaggaagc
     1381 cagatattta tgagcggctt gcttcagcct tggctcccag catttatgaa catgaagata
     1441 tcaaaaaggg aatcttactt cagctctttg gtggaacaag gaaggatttc agtcacactg
     1501 ggaggggtaa attccgtgct gagatcaaca tccttctgtg tggggaccct ggcaccagca
     1561 agtcccagct gctacagtat gtgtacaacc tggtccccag aggccagtac acgtctggaa
     1621 aaggctccag tgcggtcggc ctcaccgcct atgtgatgaa agaccctgag accaggcagc
     1681 ttgtcctcca gacaggtgcc ctcgtcctga gtgacaatgg gatatgctgc atcgatgagt
     1741 ttgacaaaat gaatgaaagc acaaggtctg tgctgcatga ggtcatggaa cagcagactc
     1801 tgtccattgc aaaggctggg atcatctgtc agctcaatgc gcgcacctct gtcctggcag
     1861 cagcaaatcc tattgagtct cagtggaatc ctaaaaaaac aaccattgaa aatatccaac
     1921 taccgcacac attgttgtca aggtttgatc tcattttcct catgctagac cctcaggatg
     1981 aggcatatga ccggcgtcta gctcatcacc tggtttcatt gtactaccaa agtgaggagc
     2041 aagtggagga ggagttcctg gacatggccg tgctgaaaga ctacattgca tatgcccata
     2101 gtaccatcat gccccgactg agtgaggagg ccagccaggc tctcattgag gcttatgtaa
     2161 acatgaggaa gattgggagt agccggggga tggtttctgc ttaccctcga cagctagagt
     2221 cattaattcg cttagcagaa gcccatgcta aagtaagatt ttcaaacaaa gttgaagcaa
     2281 ttgatgtgga agaggcaaaa cgcctccacc gggaggctct gaagcagtct gcaactgacc
     2341 ctcgtactgg cattgtggat atttctattc ttactacagg aatgagtgcc acttctcgta
     2401 aacggaaaga agaattagct gaagcattga gaaaacttat tttatctaag ggtaaaacac
     2461 cagccttaaa gtaccaacag ctgtttgagg atattcgggg acagtctgac acagcaatta
     2521 ccaaggacat gtttgaagaa gccctgcgag ctttggctga tgatgatttc ctaacagtga
     2581 ctgggaagac tgtccgcctg ctcggcgaag ggcaagggca agggcaaggg ccgggccgcg
     2641 gctacgcgta tcgatcaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
     2701 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     2761 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     2821 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttgacctac ggcgtgcagt
     2881 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     2941 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     3001 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     3061 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaagg
     3121 tctatatcac cgccgacaag cagaagaacg gcatcaaggt gaacttcaag acccgccaca
     3181 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     3241 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag
     3301 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     3361 ctctcggcat ggacgagctg tacaagtaag cggccgcgac tctagatc
//
