LOCUS       sequence-84329-e        3324 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Kuipers MA, Stasevich TJ, Sasaki T, Wilson KA, Hazelwood KL, McNally
            JG, Davidson MW, Gilbert DM
  TITLE     Highly stable loading of Mcm proteins onto chromatin in living cells
            requires replication to unload.
  JOURNAL   J Cell Biol. 2011 Jan 10;192(1):29-41. doi: 10.1083/jcb.201007111.
  PUBMED    21220507
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2011-01-10- 10"; volume: "192"; 
            issue: "1"; pages: "29-41"
COMMENT     Sequence Label: mEmerald-MCM6-C-19
FEATURES             Location/Qualifiers
     source          1..3324
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggagaa gggcaagggc aagggcaagg
      781 gccgggccgc ggctacgcgt atcgatccat ggacctcgca gcggccgcgg agcccggcgc
      841 cggaagccag cacccggagg tgcgcgacga ggtggccgag aaatgccaga agctgttcct
      901 agacttcctg gaagagttcc agggtagtga tggagaaatt aaatacttgc agtttgcaga
      961 ggagctaatt cgtcctgaga gaaacacgct ggttgtgagt tttgcagacc tggaacaatt
     1021 taaccaacaa ctttctacca ccattcagga agagttctat agagtctacc cttacctgtg
     1081 tcgagccttg aagacctttg tcaaagaccg aaaggagatc ccttttgcta aggattttta
     1141 tgttgcattc caagacctac ctactagaca caagattcgt gagctcactt catccaggat
     1201 tgggctgctc acccgaatca gtggccaggt ggtgcgcact cacccagttc accctgagct
     1261 tgtgagtgga actttcctgt gccttgactg tcaaacagtg attaaggacg tagagcagca
     1321 gttcaaatac acacagccga atatctgccg aaatccagtg tgtgccaaca ggaagaggtt
     1381 ccttctagac actaataaat caagatttgt tgattttcaa aaggttcgca ttcaagagac
     1441 tcaagcggaa cttccccggg gaagtattcc ccggagttta gaggttatcc tgagagctga
     1501 agctgtggag tcagcccaag ctggtgacag atgtgatttc acaggggcac tgattgttgt
     1561 gcctgatgtc tccaagctta gcacaccagg agcacgtgca gagactaact cccgagtcag
     1621 tggagctgat gggtatgaga cagaaggcat tcgagggctc cgggcccttg gtgtcagaga
     1681 tctgtcatac aggctggtct tccttgcctg ccatgttgca ccaaccaacc caaggtttgg
     1741 agggaaagaa ctcagagatg aggaacagac cgctgagagc attaagaacc aaatgacggt
     1801 gaaggaatgg gaaaaggtgt ttgaaatgag tcaggacaaa aacctgtacc acaatctctg
     1861 cacgagcctc ttccctacta tacatggcaa tgatgaagta aaacgcggtg tcttactgat
     1921 gttgttcggt ggtgttccta agacaacagg ggaaggaacc tctcttcgtg gggatataaa
     1981 tgtttgcatt gttggtgatc caagtacagc taagagccaa tttctcaagc acgtggacga
     2041 gttcagtccc agagctgtct ataccagtgg caaagcctcc agcgctgccg gcttaacagc
     2101 agctgtggtg agagatgaag aatctcacga atttgtcatt gaggctggag cgttgatgct
     2161 ggctgataat ggtgtctgtt gtattgatga atttgataag atggacatgc gggaccaagt
     2221 tgctattcac gaagctatgg agcagcagac catctctatc acgaaagcag gagtgaaggc
     2281 aactctgaat gccaggacat ccattttagc tgcagcaaac cctgtcagcg gacactatga
     2341 cagatcaaaa tctttgaaac agaacatcaa tctgtcagcg cccatcatgt cccgctttga
     2401 tctcttcttc attctggtgg atgaatgcaa tgaggtgaca gattatgcta ttgccaggcg
     2461 catagtagac ttgcattcaa gaattgaaga atcgatcgat cgagtttatt cccttgatga
     2521 tataagaagg taccttctct ttgcccgaca gtttaaaccc aagatttcca aagagtcaga
     2581 agacttcatt gtggagcagt acaaacgtct ccgccagagg gatggctctg gggtcaccaa
     2641 gtcctcgtgg agaatcaccg tgcgacagct tgagagcatg atccgactct cagaatcaat
     2701 ggcccgcatg cactgctgtg acgaggttca gcctaaacat gtgaaggaag cttttcgatt
     2761 actgaataaa tcaatcatcc gtgtagagac gcctgatgtc aatctagatc aagaggaaga
     2821 gatccagatg gagacggatg agggacaagg aggcgtcaat ggtcatgccg acagccctgc
     2881 ccctgtgaat aggttcaacg gctccagtga agatgccagc caggagactg tttccaagcc
     2941 ctccctgagg ctgggctttg ctgaatactg ccgaatctcg aacctcatcg tgctccacct
     3001 caggaaaatg gaagaagagg aggacgagtc ggcactaaag agaagcgagc ttgtcaactg
     3061 gtacctgaag gaaatcgaat cagagataga ctctgaagaa gagctcataa ataaaaagac
     3121 gatcattgag aaggttgttc atcgtctcac acactacgat cacgttctga ttgagctcac
     3181 ccaggctggg ctgaaaggct ccagtgaagg aagcgagagc tatgaggaag acccctacct
     3241 ggtagtcaac cctaactact tgcttgaaga ttgaggatcc accggatcta gataactgat
     3301 cataatcagc cataccacat tgag
//
