LOCUS       sequence-84331-e        3004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Kuipers MA, Stasevich TJ, Sasaki T, Wilson KA, Hazelwood KL, McNally
            JG, Davidson MW, Gilbert DM
  TITLE     Highly stable loading of Mcm proteins onto chromatin in living cells
            requires replication to unload.
  JOURNAL   J Cell Biol. 2011 Jan 10;192(1):29-41. doi: 10.1083/jcb.201007111.
  PUBMED    21220507
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2011-01-10- 10"; volume: "192"; 
            issue: "1"; pages: "29-41"
COMMENT     Sequence Label: mEmerald-MCM7-N-22
FEATURES             Location/Qualifiers
     source          1..3004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2240..2249
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2246..2248,2249..2251,2252..2965)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2291..2312)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2552..2571)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2899..2920
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcca ccatggcgct taaggactac gcgatcgaaa aagaaaaagt
       61 taagaagttc ctccaagagt tttattacga gaatgagctt ggaaagaagc agttcaagta
      121 tgggacccag ttggttcatc tggctcatcg ggaacaagtg gcactgtatg tggacctgga
      181 tgacatagct gaagatgacc ctgagttggt cgactcaatt tgcgagaatg ccaagcgcta
      241 ctcaagactg tttggtgatg ttgtgcaaga gcttttgcct gagtacaagg agaaggaggt
      301 ggtgaataag gatgtcttag atgtttacat tgaacaccgg ctgatgatgg agcagcgaag
      361 cagagaccct ggggcagtcc ggaaccccca aaaccagtat ccttctgaac tcatgcgaag
      421 atttgagttg tatttccgag gcccaagtag cagcaagcct cgagtgatcc gggaagtacg
      481 agctgactct gtggggaaat tgttaactgt gcgtggcatt gtcactcgtg tgtctgaagt
      541 caagcccagg atggtggtgg ccacatacac ttgtgatcag tgtggggcag agacctacca
      601 gccaatccag tctcccactt tcatgcctct gatcatgtgc cccagccagg agtgccagac
      661 caatcgctca ggggggcggc tgtacctaca gactcgtggc tccaaatttg ttaaattcca
      721 ggaaatgaag attcaagagc atagtgacca agttcctgtg ggaaacatcc ctcgtagcat
      781 cacagtggtt ctagaaggtg agaacacaag aattgcccag cctggtgacc atgtcagcgt
      841 cactggcatc ttcttgccag tcctgcgcac agggttccaa cagatggcac agggcttact
      901 ctcagaaact tacctggaag cccactggat tgtgaagatg acaaagagtg acgatgatgt
      961 ctctggggcc ggagagctga gctcagagga gctgaagcag attgcagagg aggatttcta
     1021 tgagaagctg gccgcttcta ttgctccgga gatctatgga catgaagatg tgaagaaggc
     1081 gctgctgctg cttctggtgg gtggtgtgga ccagtctcct caaggcatga agatcagagg
     1141 aaacatccac atctgcctta tgggagaccc tggtgtggcc aaatctcagc tcctatctta
     1201 catcgaccgt ctggcaccgc gaagtcagta cacaacaggc cgtggctcct ctggtgtagg
     1261 gctcacagca gctgtgttga gagactctgt gagtggagaa ctgactttag agggcggtgc
     1321 cctggtgctg gctgaccagg gcgtgtgctg cattgatgag tttgacaaga tggccgaggc
     1381 tgaccgcaca gccatccatg aggttatgga gcagcagacc atctccatcg ccaaggcggg
     1441 gattctcacc acgctcaatg cccgatgctc catcctggct gcggccaatc ctgcctacgg
     1501 ccgatataat ccccgccgca gtcttgagca gaacgtccag cttccggctg ctctgctttc
     1561 tcgattcgac ctcctctggc tgattcaaga ccggcccgac agagacaatg acctccggtt
     1621 ggcccagcac atcacctatg tccaccagca cagccggcag ccccctgccc aatttgaacc
     1681 tttggacatg aaacttatga gacggtacat agccatgtgc cacgagagac agcccacagt
     1741 gcctgaatct ctggctgact acatcaccgc agcatatgtg gagatgaggc gagaggcccg
     1801 ggccagtaag gatgccacct atacttctgc ccggaccctg ctggccattc tccgactttc
     1861 cactgctctg gctcgacttc ggatggtaga cattgtggag aaggaagatg taaatgaagc
     1921 cataaggttg atggagatgt cgaaggactc ccttctaggt gaaaagggac agacagctag
     1981 gacccagagg ccagcagatg tgatatttgc caccatccgt gaattggtgt cacgggggcg
     2041 gagtgtccac ttttctgagg ctgaacagcg ctgcatatct cgtggcttca caccagccca
     2101 gttccaggca gctctggatg aatatgagga gctaaatgtc tggcaggtta atacttctcg
     2161 gacacggatc acctttgtcg gcgaagggca agggcaaggg caagggccgg gccgcggcta
     2221 cgcgtatcga tcaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     2281 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     2341 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     2401 caagctgccc gtgccctggc ccaccctcgt gaccaccttg acctacggcg tgcagtgctt
     2461 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     2521 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     2581 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     2641 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaaggtcta
     2701 tatcaccgcc gacaagcaga agaacggcat caaggtgaac ttcaagaccc gccacaacat
     2761 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     2821 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
     2881 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     2941 cggcatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc agccatacca
     3001 catt
//
