LOCUS       sequence-84332-e        1986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1986)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Migfilin-C-14
FEATURES             Location/Qualifiers
     source          1..1986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttgac ctacggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      481 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc
      541 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggaagtg ctgggagcgc aagcggaagc
      781 ggaggctccg gtgcctcaaa gcctgagaag agggtggcat cgtctgtctt tatcaccctg
      841 gcacccccgc gccgcgatgt ggccgtggcg gaggaagtga ggcaggcagt ttgtgaggcc
      901 cggcgtggcc gcccctggga ggctcctgcc cccatgaaga cacccgaggc tggcttggcg
      961 gggaggccca gcccctggac aacccctggc agagctgcag ccacagtgcc ggctgcacct
     1021 atgcagctct tcaatggagg atgcccaccc cctcctcctg tcctggatgg tgaggacgtg
     1081 cttcctgacc tggacctcct cccaccccct ccaccgcccc ctccagtgct tctgccttct
     1141 gaagaggagg ctcctgctcc aatgggggcc tcactcattg cagacttaga gcagctgcac
     1201 ctgtccccgc ccccgccccc accacaggcc ccagcggagg gaccttcagt ccagcccggt
     1261 cccctcaggc ccatggagga agagctgcca cctcccccgg cagaacctgt tgagaaaggg
     1321 gcatccacag acatctgtgc cttctgctac aagaccgtgt tcccccgaga gctggctgtg
     1381 gaggccatga agaggcagta ccatgcccag tgcttcacgt gccgcacctg ccgccgccag
     1441 ctggctgggc agagcttcta ccagaaggat gggcgacccc tctgcgaacc ctgctaccag
     1501 gacacactgg agaggtgcgg caagtgtggc gaggtggtcc gggaccacat catcagggcc
     1561 ctgggccagg ccttccaccc ctcctgcttc acgtgtgtga cctgcgcccg gtgcattggg
     1621 gatgagagct ttgccctggg cagccagaac gaggtgtact gcctggacga cttctacagg
     1681 aaattcgccc ccgtctgcag catctgtgaa aatcccatca tccctcggga tgggaaagat
     1741 gccttcaaaa tcgaatgcat gggaagaaac ttccatgaaa attgctacag gtgtgaggac
     1801 tgcaggatcc tcctgtctgt cgagcccacg gaccaaggct gctaccccct gaacaaccat
     1861 ctcttctgca agccatgcca tgtgaagcgg agtgctgcgg ggtgctgctg agaattctgc
     1921 agtcgacggt accgcgggcc cgggatccac cggatctaga taactgatca taatcagcca
     1981 taccac
//
