LOCUS       sequence-84333-e        1965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1965)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Migfilin-N-16
FEATURES             Location/Qualifiers
     source          1..1965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1201..1210
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1207..1209,1210..1212,1213..1926)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1252..1273)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1513..1532)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1860..1881
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagaccgcca tggcctcaaa gcctgagaag
       61 agggtggcat cgtctgtctt tatcaccctg gcacccccgc gccgcgatgt ggccgtggcg
      121 gaggaagtga ggcaggcagt ttgtgaggcc cggcgtggcc gcccctggga ggctcctgcc
      181 cccatgaaga cacccgaggc tggcttggcg gggaggccca gcccctggac aacccctggc
      241 agagctgcag ccacagtgcc ggctgcacct atgcagctct tcaatggagg atgcccaccc
      301 cctcctcctg tcctggatgg tgaggacgtg cttcctgacc tggacctcct cccaccccct
      361 ccaccgcccc ctccagtgct tctgccttct gaagaggagg ctcctgctcc aatgggggcc
      421 tcactcattg cagacttaga gcagctgcac ctgtccccgc ccccgccccc accacaggcc
      481 ccagcggagg gaccttcagt ccagcccggt cccctcaggc ccatggagga agagctgcca
      541 cctcccccgg cagaacctgt tgagaaaggg gcatccacag acatctgtgc cttctgctac
      601 aagaccgtgt tcccccgaga gctggctgtg gaggccatga agaggcagta ccatgcccag
      661 tgcttcacgt gccgcacctg ccgccgccag ctggctgggc agagcttcta ccagaaggat
      721 gggcgacccc tctgcgaacc ctgctaccag gacacactgg agaggtgcgg caagtgtggc
      781 gaggtggtcc gggaccacat catcagggcc ctgggccagg ccttccaccc ctcctgcttc
      841 acgtgtgtga cctgcgcccg gtgcattggg gatgagagct ttgccctggg cagccagaac
      901 gaggtgtact gcctggacga cttctacagg aaattcgccc ccgtctgcag catctgtgaa
      961 aatcccatca tccctcggga tgggaaagat gccttcaaaa tcgaatgcat gggaagaaac
     1021 ttccatgaaa attgctacag gtgtgaggac tgcaggatcc tcctgtctgt cgagcccacg
     1081 gaccaaggct gctaccccct gaacaaccat ctcttctgca agccatgcca tgtgaagcgg
     1141 agtgctgcgg ggtgctgcgc atccgggggt gcgtcaggcg gcagcggatc tggaccggtc
     1201 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     1261 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     1321 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     1381 cccaccctcg tgaccacctt gacctacggc gtgcagtgct tcgcccgcta ccccgaccac
     1441 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     1501 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     1561 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1621 gggcacaagc tggagtacaa ctacaacagc cacaaggtct atatcaccgc cgacaagcag
     1681 aagaacggca tcaaggtgaa cttcaagacc cgccacaaca tcgaggacgg cagcgtgcag
     1741 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1801 aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     1861 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1921 aagtaagcgg ccgcgactct agatcataat cagccatacc acatt
//
