LOCUS       sequence-84335-e        2547 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton/Membrane, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2547)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Moesin-N-14
FEATURES             Location/Qualifiers
     source          1..2547
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1791..1800
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1797..1799,1800..1802,1803..2516)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1842..1863)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2103..2122)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2450..2471
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgcc accatgccca aaacgatcag tgtgcgtgtg accaccatgg
       61 atgcagagct ggagtttgcc atccagccca acaccaccgg gaagcagcta tttgaccagg
      121 tggtgaaaac tattggcttg agggaagttt ggttctttgg tctgcagtac caggacacta
      181 aaggtttctc cacctggctg aaactcaata agaaggtgac tgcccaggat gtgcggaagg
      241 aaagccccct gctctttaag ttccgtgcca agttctaccc tgaggatgtg tccgaggaat
      301 tgattcagga catcactcag cgcctgttct ttctgcaagt gaaagagggc attctcaatg
      361 atgatattta ctgcccgcct gagaccgctg tgctgctggc ctcgtatgct gtccagtcta
      421 agtatggcga cttcaataag gaagtgcata agtctggcta cctggccgga gacaagttgc
      481 tcccgcagag agtcctggaa cagcacaaac tcaacaagga ccagtgggag gagcggatcc
      541 aggtgtggca tgaggaacac cgtggcatgc tcagggagga tgctgtcctg gaatatctga
      601 agattgctca agatctggag atgtatggtg tgaactactt cagcatcaag aacaagaaag
      661 gctcagagct gtggctgggg gtggatgccc tgggtctcaa catctatgag cagaatgaca
      721 gactaactcc caagataggc ttcccctgga gtgaaatcag gaacatctct ttcaatgata
      781 agaaatttgt catcaagccc attgacaaaa aagccccgga cttcgtcttc tatgctcccc
      841 ggctgcggat taacaagcgg atcttggcct tgtgcatggg gaaccatgaa ctatacatgc
      901 gccgtcgcaa gcctgatacc attgaggtgc agcagatgaa ggcacaggcc cgggaggaga
      961 agcaccagaa gcagatggag cgtgctatgc tggaaaatga gaagaagaag cgtgaaatgg
     1021 cagagaagga gaaagagaag attgaacggg agaaggagga gctgatggag aggctgaagc
     1081 agatcgagga acagactaag aaggctcagc aagaactgga agaacagacc cgtagggctc
     1141 tggaacttga gcaggaacgg aagcgtgccc agagcgaggc tgaaaagctg gccaaggagc
     1201 gtcaagaagc tgaagaggcc aaggaggcct tgctgcaggc ctcccgggac cagaaaaaga
     1261 ctcaggaaca gctggccttg gaaatggcag agctgacagc tcgaatctcc cagctggaga
     1321 tggcccgaca gaagaaggag agtgaggctg tggagtggca gcagaaggcc cagatggtac
     1381 aggaagactt ggagaagacc cgtgctgagc tgaagactgc catgagtaca cctcatgtgg
     1441 cagagcctgc tgagaatgag caggatgagc aggatgagaa tggggcagag gctagtgctg
     1501 acctacgggc tgatgctatg gccaaggacc gcagtgagga ggaacgtacc actgaggcag
     1561 agaagaatga gcgtgtgcag aagcacctga aggccctcac ttcggagctg gccaatgcca
     1621 gagatgagtc caagaagact gccaatgaca tgatccatgc tgagaacatg cgactgggcc
     1681 gagacaaata caagaccctg cgccagatcc ggcagggcaa caccaagcag cgcattgacg
     1741 aatttgagtc tatggcatcc ggcggtagcg gcggtggcag cggaccggtc gccaccatgg
     1801 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     1861 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     1921 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     1981 tgaccacctt gacctacggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc
     2041 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     2101 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     2161 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     2221 tggagtacaa ctacaacagc cacaaggtct atatcaccgc cgacaagcag aagaacggca
     2281 tcaaggtgaa cttcaagacc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     2341 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     2401 tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     2461 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagcgg
     2521 ccgcgactct agatcataat cagccat
//
