LOCUS       sequence-84336-e        3104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosomes, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3104)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-MPO-C-18
FEATURES             Location/Qualifiers
     source          1..3104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(25..27,28..30,31..741)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(70..91)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(331..350)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     678..699
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
       61 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      121 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      181 aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt gcagtgcttc
      241 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      301 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      361 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      421 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caaggtctat
      481 atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg ccacaacatc
      541 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      601 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc
      661 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      721 ggcatggacg agctgtacaa gtccggactc agatctcgag gaagcggcgg atctgggtcc
      781 ggcagctccg gcagcggggt tcccttcttc tcttctctca gatgcatggt ggacttagga
      841 ccttgctggg ctgggggtct cactgcagag atgaagctgc ttctggccct agcagggctc
      901 ctggccattc tggccacgcc ccagccctct gaaggtgctg ctccagctgt cctgggggag
      961 gtggacacct cgttggtgct gagctccatg gaggaggcca agcagctggt ggacaaggcc
     1021 tacaaggagc ggcgggaaag catcaagcag cggcttcgca gcggctcagc cagccccatg
     1081 gaactcctat cctacttcaa gcagccggtg gcagccacca ggacggcggt gagggccgct
     1141 gactacctgc acgtggctct agacctgctg gagaggaagc tgcggtccct gtggcgaagg
     1201 ccattcaatg tcactgatgt gctgacgccc gcccagctga atgtgttgtc caagtcaagc
     1261 ggctgcgcct accaggacgt gggggtgact tgcccggagc aggacaaata ccgcaccatc
     1321 accgggatgt gcaacaacag acgcagcccc acgctggggg cctccaaccg tgcctttgtg
     1381 cgctggctgc cggcggagta tgaggacggc ttctctcttc cctacggctg gacgcccggg
     1441 gtcaagcgca acggcttccc ggtggctctg gctcgcgcgg tctccaacga gatcgtgcgc
     1501 ttccccactg atcagctgac tccggaccag gagcgctcac tcatgttcat gcaatggggc
     1561 cagctgttgg accacgacct cgacttcacc cctgagccgg ccgcccgggc ctccttcgtc
     1621 actggcgtca actgcgagac cagctgcgtt cagcagccgc cctgcttccc gctcaagatc
     1681 ccgcccaatg acccccgcat caagaaccaa gccgactgca tcccgttctt ccgctcctgc
     1741 ccggcttgcc ccgggagcaa catcaccatc cgcaaccaga tcaacgcgct cacttccttc
     1801 gtggacgcca gcatggtgta cggcagcgag gagcccctgg ccaggaacct gcgcaacatg
     1861 tccaaccagc tggggctgct ggccgtcaac cagcgcttcc aagacaacgg ccgggccctg
     1921 ctgccctttg acaacctgca cgatgacccc tgtctcctca ccaaccgctc agcgcgcatc
     1981 ccctgcttcc tggcagggga cacccgttcc agtgagatgc ccgagctcac ctccatgcac
     2041 accctcttac ttcgggagca caaccggctg gccacagagc tcaagagcct gaaccctagg
     2101 tgggatgggg agaggctcta ccaggaagcc cggaagatcg tgggggccat ggtccagatc
     2161 atcacttacc gggactacct gcccctggtg ctggggccaa cggccatgag gaagtacctg
     2221 cccacgtacc gttcctacaa tgactcagtg gacccacgca tcgccaacgt cttcaccaat
     2281 gccttccgct acggccacac cctcatccaa cccttcatgt tccgcctgga caatcggtac
     2341 cagcccatgg aacccaaccc ccgtgtcccc ctcagcaggg tcttttttgc ctcctggagg
     2401 gtcgtgctgg aaggtggcat tgaccccatc ctccggggcc tcatggccac ccctgccaag
     2461 ctgaatcgtc agaaccaaat tgcagtggat gagatccggg agcgattgtt tgagcaggtc
     2521 atgaggattg ggctggacct gcctgctctg aacatgcagc gcagcaggga ccacggcctc
     2581 ccaggataca atgcctggag gcgcttctgt gggctcccgc agcctgaaac tgtgggccag
     2641 ctgggcacgg tgctgaggaa cctgaaattg gcgaggaaac tgatggagca gtatggcacg
     2701 cccaacaaca tcgacatctg gatgggcggc gtgtccgagc ctctgaagcg caaaggccgc
     2761 gtgggcccac tcctcgcctg catcatcggt acccagttca ggaagctccg ggatggtgat
     2821 cggttttggt gggagaacga gggtgtgttc agcatgcagc agcgacaggc cctggcccag
     2881 atctcattgc cccggatcat ctgcgacaac acaggcatca ccaccgtgtc taagaacaac
     2941 atcttcatgt ccaactcata tccccgggac tttgtcaact gcagtacact tcctgcattg
     3001 aacctggctt cctggaggga agcctcctag aagcttcgaa ttctgcagtc gacggtaccg
     3061 cgggcccggg atccaccgga tctagataac tgatcataat cagc
//
