LOCUS       sequence-84338-e        4777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton , Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4777)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Myopalladin-C-14
FEATURES             Location/Qualifiers
     source          1..4777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(26..28,29..31,32..742)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(71..92)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     679..700
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
       61 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      121 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      181 caagctgccc gtgccctggc ccaccctcgt gaccaccttg acctacggcg tgcagtgctt
      241 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      301 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      361 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      421 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaaggtcta
      481 tatcaccgcc gacaagcaga agaacggcat caaggtgaac ttcaagaccc gccacaacat
      541 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
      601 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
      661 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
      721 cggcatggac gagctgtaca agtccggaag tgctgggagc gcaagcggaa gcggaggctc
      781 cggtcaagac gacagcatag aagcttctac ttccatatct cagcttctaa gagagagcta
      841 tttagctgaa accagacatc ggggaaacaa tgagaggagt cgagcggagc cctcctccaa
      901 cccttgccat ttcggcagtc cttctggggc cgctgaagga ggcggaggcc aagatgacct
      961 tccagatctt tcagcctttc tgagccaaga agaattagac gaaagtgtca atttggcaag
     1021 actggccatc aattacgacc ctttggagaa ggcagatgaa actcaagcta gaaaacgact
     1081 ttctcctgat cagatgaaac actcacctaa tttaagtttt gagcctaact tctgccagga
     1141 taaccctcga agtcccacca gctctaaaga aagcccccag gaggcaaaaa ggccacagta
     1201 ttgttctgaa acccagtcca aaaaagtatt tttaaataag gctgccgact tcattgaaga
     1261 gctatcctcc cttttcaaat cccacagctc caaaaggatt agacctcgtg cctgcaaaaa
     1321 ccacaagagt aaactggaat ctcaaaacaa agttatgcag gaaaacagct ccagtttctc
     1381 agatctgtca gaaagacgag aaagatcttc tgttcccatc cctatccctg cggataccag
     1441 ggataatgaa gtgaatcacg ccctggaaca gcaggaagcc aagaggcgtg aagcggagca
     1501 ggctgccagt gaggcggctg gtggagacac tacaccaggg tcttcccctt catctctgta
     1561 ctatgaagaa cctctggggc aacctccccg gttcactcaa aagttacgga gcagagaagt
     1621 tccagaagga actcgagtac agttggattg catagtggta ggaattccac cacctcaagt
     1681 aaggtggtac tgtgaaggca aggagcttga aaattcccca gatattcaca tcgcccaggc
     1741 aggaaatctg cactcactga ccattgcgga agcctttgaa gaggacacag gacgctattc
     1801 ctgctttgct tctaacatct atgggacaga ttcgacttct gctgagattt atatagaagg
     1861 ggtttcttct tctgactcag aaggcgaccc taacaaggaa gagatgaatc gaatccagaa
     1921 gccaaatgag gtgtcatctc ctcccactac ctctgcagtc attcctccag cagtacccca
     1981 agcccagcat ttggtggccc aacctcgtgt ggcaaccatc cagcagtgtc agagccccac
     2041 caattacttg cagggattgg atggaaaacc tatcattgca gctcctgtgt ttacaaagat
     2101 gctacaaaat ttgtcagctt ctgagggtca gctggttgtc tttgaatgca gagtaaaagg
     2161 agctccatct cctaaggttg agtggtatag agaagggact ttaatagaag attctccaga
     2221 ttttaggatt ttacagaaaa aacctcgatc catggcagag ccagaggaga tttgcacctt
     2281 ggtcattgct gaggtgtttg cagaagattc tgggtgcttc acatgtactg caagcaacaa
     2341 atacggcaca gtgtcaagca ttgcacagct gcacgtgaga ggaaatgagg acctcagcaa
     2401 caacgggtct cttcactcag ccaactccac caccaacctg gcagctattg agccacagcc
     2461 ctccccaccc cactcagagc ctccatctgt ggaacaaccc cccaaaccca aactcgaggg
     2521 ggttctggtg aaccacaatg agccccggtc cagctccagg attgggcttc gtgtgcactt
     2581 caacctgcct gaagatgaca aaggaagtga agcatcctcc gaggctggtg tggtgaccac
     2641 cagacagacc aggcccgatt ctttgcagga gaggttcaac ggacaggcaa caaaaacccc
     2701 agagccttct tcccccgtga aagagccccc tccagttctg gccaaaccca aacttgattc
     2761 cactcagtta caacagcttc ataaccaagt cttactggaa caacaccaat tgcaaaaccc
     2821 acctccttca tctcctaagg agtttccttt cagcatgact gttttgaact ccaatgctcc
     2881 cccagcggtg acaacatcca gtaagcaggt gaaggctcct tcatcacaga cgttcagctt
     2941 ggcccggccg aagtatttct tcccctccac gaacaccacc gcagcaactg tggccccttc
     3001 cagctctccg gtgttcactt tgagcagcac tcctcaaact attcagagga cagtgagcaa
     3061 agaaagcctc ttagtgtctc acccctctgt gcaaaccaaa tctccaggag ggctttccat
     3121 ccaaaatgag ccactcccac caggcccaac agaaccaaca ccaccaccat tcacattttc
     3181 catccccagc ggaaaccagt ttcagccccg ctgtgtgtcc ccaattcctg tctctcctac
     3241 cagccggatt cagaacccag tggctttcct cagctctgtt ctgccttctc tccctgccat
     3301 cccacccaca aatgccatgg ggctgcctag aagtgcacca tccatgccat cccagggatt
     3361 agcgaagaaa aatacaaagt ctcctcaacc agtgaatgat gataacattc gtgaaactaa
     3421 gaacgcagtg attcgagact tgggaaaaaa aataactttc agtgatgtca gaccaaacca
     3481 gcaggagtac aaaatttcaa gctttgagca gaggctgatg aatgaaatag agtttcgctt
     3541 ggaacgtact cctgttgatg aatcagatga tgaaattcaa catgatgaga tccccacggg
     3601 caagtgtatt gctcccatct ttgacaagag actcaagcac ttccgggtca cagaaggctc
     3661 tccagtcaca ttcacctgca aaattgttgg gatacctgtt ccaaaggttt actggttcaa
     3721 agatgggaag cagatttcta agagaaatga gcactgcaaa atgaggcgag aaggagatgg
     3781 gacatgttct ctgcacattg aatccactac cagtgatgac gatggcaact acaccatcat
     3841 ggcagccaac ccccagggga gaatcagctg ttctggccac ttgatggtac aaagtttgcc
     3901 cattcgcagt cggctaacct ctgctggtca gtctcacagg ggaagatccc gagtgcaaga
     3961 aagagacaaa gagcccctac aggaacgctt tttccgacca catttcctgc aggctcctgg
     4021 ggatatggta gctcatgagg ggcgcctctg tcggctggac tgtaaggtga gtggtttacc
     4081 gcccccggag ctgacatggc tactcaatgg ccaacctgtg ctaccagatg cctcccacaa
     4141 gatgctggtc agggagaccg gagtccactc tctgctcatt gacccactca ctcagcgcga
     4201 cgcagggacc tataagtgca tcgctaccaa caaaaccggg cagaattctt ttagtctgga
     4261 gctctctgta gtagccaaag aggtgaagaa agcacctgtg atcctggaga aactacagaa
     4321 ctgcggtgtt cccgaaggcc accccgtgag actggagtgc cgcgtgatag gcatgccccc
     4381 acctgtgttc tactggaaga aagacaatga gaccatccct tgcaccagag agaggatcag
     4441 tatgcaccag gacacaacag ggtatgcctg ccttctcatt cagccagcca agaaatcaga
     4501 cgctggatgg tacacgttgt cagccaagaa tgaagccggc atcgtgtcgt gcactgccag
     4561 gctggatata tacgctcagt ggcaccatca gatcccaccg cccatgtctg tccggcccag
     4621 tggcagtcgc tacggatctc tcaccagtaa aggacttgac atattttctg ccttttcctc
     4681 catggaaagc acgatggtgt attcatgctc ttctcggagt gtagtggaga gtgatgaact
     4741 ttaaggatcc accggatcta gataactgat cataatc
//
