LOCUS       sequence-84339-e        4780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton , Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4780)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Myopalladin-N-18
FEATURES             Location/Qualifiers
     source          1..4780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4030..4039
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4036..4038,4039..4041,4042..4755)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4081..4102)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4689..4710
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagatccg ctagcgccac catgcaagac gacagcatag aagcttctac ttccatatct
       61 cagcttctaa gagagagcta tttagctgaa accagacatc ggggaaacaa tgagaggagt
      121 cgagcggagc cctcctccaa cccttgccat ttcggcagtc cttctggggc cgctgaagga
      181 ggcggaggcc aagatgacct tccagatctt tcagcctttc tgagccaaga agaattagac
      241 gaaagtgtca atttggcaag actggccatc aattacgacc ctttggagaa ggcagatgaa
      301 actcaagcta gaaaacgact ttctcctgat cagatgaaac actcacctaa tttaagtttt
      361 gagcctaact tctgccagga taaccctcga agtcccacca gctctaaaga aagcccccag
      421 gaggcaaaaa ggccacagta ttgttctgaa acccagtcca aaaaagtatt tttaaataag
      481 gctgccgact tcattgaaga gctatcctcc cttttcaaat cccacagctc caaaaggatt
      541 agacctcgtg cctgcaaaaa ccacaagagt aaactggaat ctcaaaacaa agttatgcag
      601 gaaaacagct ccagtttctc agatctgtca gaaagacgag aaagatcttc tgttcccatc
      661 cctatccctg cggataccag ggataatgaa gtgaatcacg ccctggaaca gcaggaagcc
      721 aagaggcgtg aagcggagca ggctgccagt gaggcggctg gtggagacac tacaccaggg
      781 tcttcccctt catctctgta ctatgaagaa cctctggggc aacctccccg gttcactcaa
      841 aagttacgga gcagagaagt tccagaagga actcgagtac agttggattg catagtggta
      901 ggaattccac cacctcaagt aaggtggtac tgtgaaggca aggagcttga aaattcccca
      961 gatattcaca tcgcccaggc aggaaatctg cactcactga ccattgcgga agcctttgaa
     1021 gaggacacag gacgctattc ctgctttgct tctaacatct atgggacaga ttcgacttct
     1081 gctgagattt atatagaagg ggtttcttct tctgactcag aaggcgaccc taacaaggaa
     1141 gagatgaatc gaatccagaa gccaaatgag gtgtcatctc ctcccactac ctctgcagtc
     1201 attcctccag cagtacccca agcccagcat ttggtggccc aacctcgtgt ggcaaccatc
     1261 cagcagtgtc agagccccac caattacttg cagggattgg atggaaaacc tatcattgca
     1321 gctcctgtgt ttacaaagat gctacaaaat ttgtcagctt ctgagggtca gctggttgtc
     1381 tttgaatgca gagtaaaagg agctccatct cctaaggttg agtggtatag agaagggact
     1441 ttaatagaag attctccaga ttttaggatt ttacagaaaa aacctcgatc catggcagag
     1501 ccagaggaga tttgcacctt ggtcattgct gaggtgtttg cagaagattc tgggtgcttc
     1561 acatgtactg caagcaacaa atacggcaca gtgtcaagca ttgcacagct gcacgtgaga
     1621 ggaaatgagg acctcagcaa caacgggtct cttcactcag ccaactccac caccaacctg
     1681 gcagctattg agccacagcc ctccccaccc cactcagagc ctccatctgt ggaacaaccc
     1741 cccaaaccca aactcgaggg ggttctggtg aaccacaatg agccccggtc cagctccagg
     1801 attgggcttc gtgtgcactt caacctgcct gaagatgaca aaggaagtga agcatcctcc
     1861 gaggctggtg tggtgaccac cagacagacc aggcccgatt ctttgcagga gaggttcaac
     1921 ggacaggcaa caaaaacccc agagccttct tcccccgtga aagagccccc tccagttctg
     1981 gccaaaccca aacttgattc cactcagtta caacagcttc ataaccaagt cttactggaa
     2041 caacaccaat tgcaaaaccc acctccttca tctcctaagg agtttccttt cagcatgact
     2101 gttttgaact ccaatgctcc cccagcggtg acaacatcca gtaagcaggt gaaggctcct
     2161 tcatcacaga cgttcagctt ggcccggccg aagtatttct tcccctccac gaacaccacc
     2221 gcagcaactg tggccccttc cagctctccg gtgttcactt tgagcagcac tcctcaaact
     2281 attcagagga cagtgagcaa agaaagcctc ttagtgtctc acccctctgt gcaaaccaaa
     2341 tctccaggag ggctttccat ccaaaatgag ccactcccac caggcccaac agaaccaaca
     2401 ccaccaccat tcacattttc catccccagc ggaaaccagt ttcagccccg ctgtgtgtcc
     2461 ccaattcctg tctctcctac cagccggatt cagaacccag tggctttcct cagctctgtt
     2521 ctgccttctc tccctgccat cccacccaca aatgccatgg ggctgcctag aagtgcacca
     2581 tccatgccat cccagggatt agcgaagaaa aatacaaagt ctcctcaacc agtgaatgat
     2641 gataacattc gtgaaactaa gaacgcagtg attcgagact tgggaaaaaa aataactttc
     2701 agtgatgtca gaccaaacca gcaggagtac aaaatttcaa gctttgagca gaggctgatg
     2761 aatgaaatag agtttcgctt ggaacgtact cctgttgatg aatcagatga tgaaattcaa
     2821 catgatgaga tccccacggg caagtgtatt gctcccatct ttgacaagag actcaagcac
     2881 ttccgggtca cagaaggctc tccagtcaca ttcacctgca aaattgttgg gatacctgtt
     2941 ccaaaggttt actggttcaa agatgggaag cagatttcta agagaaatga gcactgcaaa
     3001 atgaggcgag aaggagatgg gacatgttct ctgcacattg aatccactac cagtgatgac
     3061 gatggcaact acaccatcat ggcagccaac ccccagggga gaatcagctg ttctggccac
     3121 ttgatggtac aaagtttgcc cattcgcagt cggctaacct ctgctggtca gtctcacagg
     3181 ggaagatccc gagtgcaaga aagagacaaa gagcccctac aggaacgctt tttccgacca
     3241 catttcctgc aggctcctgg ggatatggta gctcatgagg ggcgcctctg tcggctggac
     3301 tgtaaggtga gtggtttacc gcccccggag ctgacatggc tactcaatgg ccaacctgtg
     3361 ctaccagatg cctcccacaa gatgctggtc agggagaccg gagtccactc tctgctcatt
     3421 gacccactca ctcagcgcga cgcagggacc tataagtgca tcgctaccaa caaaaccggg
     3481 cagaattctt ttagtctgga gctctctgta gtagccaaag aggtgaagaa agcacctgtg
     3541 atcctggaga aactacagaa ctgcggtgtt cccgaaggcc accccgtgag actggagtgc
     3601 cgcgtgatag gcatgccccc acctgtgttc tactggaaga aagacaatga gaccatccct
     3661 tgcaccagag agaggatcag tatgcaccag gacacaacag ggtatgcctg ccttctcatt
     3721 cagccagcca agaaatcaga cgctggatgg tacacgttgt cagccaagaa tgaagccggc
     3781 atcgtgtcgt gcactgccag gctggatata tacgctcagt ggcaccatca gatcccaccg
     3841 cccatgtctg tccggcccag tggcagtcgc tacggatctc tcaccagtaa aggacttgac
     3901 atattttctg ccttttcctc catggaaagc acgatggtgt attcatgctc ttctcggagt
     3961 gtagtggaga gtgatgaact tagtcgcgga ggcagtggtg gggtaccgcg ggcccgggat
     4021 ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     4081 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     4141 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     4201 gtgccctggc ccaccctcgt gaccaccttg acctacggcg tgcagtgctt cgcccgctac
     4261 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     4321 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     4381 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     4441 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaaggtcta tatcaccgcc
     4501 gacaagcaga agaacggcat caaggtgaac ttcaagaccc gccacaacat cgaggacggc
     4561 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     4621 ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag
     4681 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     4741 gagctgtaca agtaagcggc cgcgactcta gatcataatc
//
