LOCUS       sequence-84343-e        6825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Ebrahim S, Fujita T, Millis BA, Kozin E, Ma X, Kawamoto S, Baird MA,
            Davidson M, Yonemura S, Hisa Y, Conti MA, Adelstein RS, Sakaguchi H,
            Kachar B
  TITLE     NMII forms a contractile transcellular sarcomeric network to 
            regulate apical cell junctions and tissue geometry.
  JOURNAL   Curr Biol. 2013 Apr 22;23(8):731-6. doi: 10.1016/j.cub.2013.03.039. 
            Epub 2013 Apr 4.
  PUBMED    23562268
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2013.03.039"; journalName: "Curr Biol"; date: 
            "2013-04-22- 22"; volume: "23"; issue: "8"; pages: "731-6"
COMMENT     Sequence Label: mEmerald-MyosinIIC-C-18
FEATURES             Location/Qualifiers
     source          1..6825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(27..29,30..32,33..743)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(72..93)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(333..352)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     680..701
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
       61 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      121 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      181 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt gacctacggc gtgcagtgct
      241 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      301 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      361 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      421 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaaggtct
      481 atatcaccgc cgacaagcag aagaacggca tcaaggtgaa cttcaagacc cgccacaaca
      541 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      601 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc
      661 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
      721 tcggcatgga cgagctgtac aagtccggaa gcggcggtgg aggcagcgca tccggcggga
      781 gcggtggatc cagcggggct gcagtgacca tgtccgtgtc tgggaggaag gtagcctcca
      841 ggccaggccc ggtgcctgag gcagcccaat cgttcctcta cgcgccccgg acgccaaatg
      901 taggtggccc tggagggcca caggtggagt ggacagcccg gcgcatggtg tgggtgccct
      961 cggaactgca tgggttcgag gcagcagccc tgcgggatga aggggaggag gaggcagaag
     1021 tggagctggc ggagagtggg cgccgcctgc ggctgcccag ggaccagatc cagcgcatga
     1081 acccacccaa gttcagcaag gcagaagata tggctgagct cacctgcctc aacgaggcct
     1141 cggtcctgca caacctgcga gaacgctact actccgggct catttatacc tactctggcc
     1201 tcttctgtgt ggtcattaac ccatacaagc agctgcccat ctacacggag gccattgttg
     1261 aaatgtaccg gggcaagaag cgccatgagg tgccacctca cgtgtatgct gtgacggagg
     1321 gcgcgtaccg cagcatgctt caggatcgtg aggatcaatc cattctctgc acgggagagt
     1381 ctggcgctgg gaagacggag aacaccaaga aggtcatcca gtacctggcc catgtggcat
     1441 catctccaaa gggcaggaag gagcctggtg tccctgcctc cgtcagcacc atgtcttatg
     1501 gggagctaga gcgtcagctt cttcaagcca accccatcct agaggccttt ggcaatgcca
     1561 agacagtgaa gaacgacaac tcttcccgat ttggcaaatt catccgcatc aactttgata
     1621 ttgctggcta catcgtggga gcaaacatcg agacctatct gttggagaag tcccgggcca
     1681 tcagacaggc caaggatgaa tgcagcttcc atatcttcta ccagctgcta gggggcgctg
     1741 gggagcagct aaaagctgac ctccttctgg agccctgttc ccattatcgc ttcctgacca
     1801 atgggccctc atcgtccccg ggccaggagc gtgagttatt ccaggagacc ctggagtccc
     1861 tgcgtgtgct gggcctcctc ccagaagaga tcactgccat gctgcgcact gtctctgctg
     1921 tcctccagtt tggcaacatt gtcctgaaga aagagcgcaa tacggaccaa gccaccatgc
     1981 ctgacaacac agctgcccag aagctttgcc gcctcttggg actcggagtg accgacttct
     2041 ccagagccct tctcacaccc cgcatcaaag tgggccgaga ttatgttcag aaagcacaaa
     2101 ccaaggagca ggctgacttt gcgctggagg ctctggccaa agctacctat gagcgcctgt
     2161 tccgctggct ggttctgcgg ctcaaccgtg ccctggacag aagcccgcgg cagggtgcct
     2221 ccttcctggg catcctggac atcgcgggct ttgagatctt ccagctgaac tccttcgagc
     2281 agctgtgcat caactacacc aacgagaagc tacagcagct attcaaccac accatgttcg
     2341 tgctggagca ggaggagtac cagcgagagg gcatcccctg gaccttccta gacttcgggt
     2401 tggacctgca accttgcatc gacctcattg agcgtccggc caaccctcca ggtctcctgg
     2461 ccctgctgga cgaggagtgc tggttcccca aggccacgga caagtctttt gtggagaagg
     2521 tcgcccagga gcagggcagc caccccaaat tccagcgccc caggaacctg cgagatcagg
     2581 ccgacttcag cgtcctgcac tacgccggca aggttgacta caaagccagt gagtggctga
     2641 tgaagaacat ggacccactg aatgacaatg tggccgcctt gcttcaccag agcacggatc
     2701 gtctcacagc tgagatctgg aaggatgtgg agggcatcgt ggggctggag caagtaagca
     2761 gccttggaga tggcccaccg ggaggccgcc cccgccgtgg aatgtttcgg actgtggggc
     2821 agctctacaa agaatccctg agccgcctca tggccacgct cagcaacacc aaccctagtt
     2881 ttgtccgctg catcgttccc aatcatgaga agagggctgg aaagctggag ccgcgcctgg
     2941 tgctggacca actccgttgt aacggggtcc tcgagggtat acgcatctgt cgccaaggct
     3001 tccccaaccg catcctcttc caggagttcc gacagcgcta tgaaatcctc accccgaacg
     3061 ctattcccaa gggcttcatg gacggcaaac aggcctgtga gaagatgatc caggccctgg
     3121 agctagaccc caacctgtac cgtgttggcc aaagcaagat cttcttccgg gcaggggtcc
     3181 tggcccagct ggaggaggag cgggacctga aagtcaccga catcatagtg tctttccagg
     3241 cagcggcacg gggctacctg gcccgtaggg ctttccagag acggcagcag cagcagagtg
     3301 ctctgagggt gatgcagaga aactgtgctg cctacctcaa gctcaggaac tggcagtggt
     3361 ggaggctgtt catcaaggtg aagcccctgc tgcaggtgac acggcaggat gaggtgctgc
     3421 aggcgcgcgc ccaggagctg cagaaagttc aggagctgca gcagcagagc gctcgtgaag
     3481 tgggggaact gcagggtcga gtggcacagc tagaggagga gcgcacgcgc ctggctgagc
     3541 agcttcgagc agaagccgag ctctgctctg aggccgagga gacgcgggcg cgactggctg
     3601 cccggaagca ggagctggag ctggtggtga cagagctgga ggcacgagtg ggcgaggaag
     3661 aagagtgcag ccggcagctg cagagtgaga agaagaggct gcagcagcat atccaggagc
     3721 tagagagcca cctggaagct gaggagggtg cccggcagaa gctacagctg gagaaggtga
     3781 ccacagaggc caagatgaag aaatttgagg aggacctgct gctcctggag gaccagaatt
     3841 ccaagctgag caaggagcgg aggctgctgg aggagcggct ggctgagttc tcctcacagg
     3901 cagcagaaga ggaagagaaa gtcaaaagtc tcaacaagct gaggctcaaa tatgaagcca
     3961 caatctcaga catggaagac cggctgaaga aggaggagaa gggacgccag gaactagaga
     4021 agctgaagcg acggctggac ggggagagct cagagcttca ggagcagatg gtggagcaga
     4081 agcagagggc agaggaactg ctcgcacagc tgggccgcaa ggaggatgag ctgcaggccg
     4141 ccctgctcag ggcagaggaa gagggtggtg cccgtgccca gttgctcaag tccctgcgag
     4201 aggcacaggc tggccttgct gaggctcagg aggacctgga agctgagcgg gtagccaggg
     4261 ccaaggcgga gaagcagcgc cgggacctgg gcgaggagtt ggaggcccta cgtggggagc
     4321 tcgaggacac tctggattcc accaacgccc agcaggagct gcggtccaag agggagcagg
     4381 aggtgacaga gctgaagaaa gcattggaag aggagtcccg tgcccatgag gtgtccatgc
     4441 aggagctgag acagaggcat agccaggcac tggtggagat ggccgagcag ttggagcaag
     4501 cccggagggg caaaggtgtg tgggagaaga ctcggctatc cctggaggct gaggtgtccg
     4561 agctgaaggc cgagctgagc agcctgcaga cctcgagaca ggagggtgag cagaagaggc
     4621 gccgcctgga gtcccagcta caggaggtcc agggccgatc cagtgattcg gagcgggctc
     4681 ggtctgaggc tgctgagaag ctgcagagag cccaggcgga acttgagagc gtgtccacag
     4741 ccctgagtga ggcggagtcc aaagccatca ggctgggcaa ggagctgagc agtgcagagt
     4801 cccagctgca tgacacccag gaactgcttc aggaggagac cagggcaaag ctggccttgg
     4861 ggtcccgtgt gcgtgcccta gaggccgagg cggcggggct tcgggagcag atggaagagg
     4921 aggtggttgc cagggaacgg gctggccggg agctgcagag cacgcaggcc cagctctctg
     4981 aatggcggcg ccgccaggaa gaagaggctg cggtgctgga ggctggggag gaggctcggc
     5041 gccgtgcagc ccgggaggca gagaccctga cccagcgcct ggcagaaaag actgaggctg
     5101 tagaacgact ggagcgagcc cggcgccgac tgcagcagga gttggacgat gccactgtgg
     5161 atctggggca gcagaagcag ctcctgagca cactggagaa gaagcagcgg aaatttgacc
     5221 agctcctggc agaggagaag gctgcagttc tacgggctgt ggaagaccgt gaacggatag
     5281 aggccgaagg ccgggagcga gaggcccggg ccctgtcgct gacccgggcc ctggaagagg
     5341 agcaggaggc ccgggaggag ctggagaggc agaaccgtgc tctgagggct gagctggaag
     5401 cactgctgag cagcaaggat gacgtgggca agaacgtgca cgagctggag cgagcccgta
     5461 aggcggctga acaggcagcc agtgacctgc ggacacaggt gacagaattg gaggatgagc
     5521 tgacagccgc agaggatgcc aagctgcgcc tggaggtgac tgtgcaggct ctgaaggctc
     5581 aacatgaacg cgacctgcag ggccgcgatg atgccggtga ggagaggcgg aggcagctgg
     5641 ccaagcagct aagagacgca gaggtagagc gcgatgagga acggaagcag agggcactgg
     5701 ctatggctgc ccgcaagaag ctggagctgg aactggagga gttgaaggcg cagacatctg
     5761 ctgctgggca gggcaaggaa gaggcagtga agcagctgaa gaagatgcag gtccagatga
     5821 aggagctgtg gcgggaggta gaggagacgc gtagctcccg cgacgagatg tttaccctga
     5881 gcagggaaaa tgagaagaag ctcaaggggc tggaagctga ggtgctgcgt ctgcaagagg
     5941 aacttgctgc ctcagaccga gcccggaggc aggcccagca agacagagac gagatggcag
     6001 aggaggtggc cagtggcaat cttagcaagg cagccaccct ggaggaaaaa cggcagctgg
     6061 aggggcgact gagccagttg gaagaggagc tggaggaaga acagaacaac tcggagctgc
     6121 tcaaggacca ttaccgaaag ctagtgctac aggtcgagtc cctcaccaca gaactgtctg
     6181 ccgaacgaag tttctcagcc aaggccgaga gtggacggca gcagctggag cggcagatcc
     6241 aggaactgcg ggcccgcttg ggtgaagagg atgctggagc ccgagccagg cagaaaatgc
     6301 tgatcgctgc tctggagtct aaactggccc aggcagagga gcagctggag caggagagca
     6361 gggagcgcat cctctctggc aagctggtac gcagagctga gaagcggctg aaggaggtag
     6421 ttcttcaggt ggatgaagag cgcagggtgg ctgaccaggt ccgggaccag ctggagaaaa
     6481 gcaacctccg gctgaagcag ctcaagaggc agctggagga ggcagaggag gaggcatctc
     6541 gggcacaggc tggtcggagg cggctgcagc gggagctgga ggacgtcact gagtctgcag
     6601 aatccatgaa ccgggaggtg accacgctga ggaacaggct ccggcgtggc ccacttacat
     6661 tcaccacacg gactgtgcgc caggtgttcc ggctggaaga gggcgtggct tctgacgagg
     6721 aagaggctga aggagctgaa cctggctctg caccaggcca ggagccggag gctccgcccc
     6781 ctgccacacc ccaatgagga tccaccggat ctagataact gatca
//
