LOCUS       sequence-84344-e        6822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Ebrahim S, Fujita T, Millis BA, Kozin E, Ma X, Kawamoto S, Baird MA,
            Davidson M, Yonemura S, Hisa Y, Conti MA, Adelstein RS, Sakaguchi H,
            Kachar B
  TITLE     NMII forms a contractile transcellular sarcomeric network to 
            regulate apical cell junctions and tissue geometry.
  JOURNAL   Curr Biol. 2013 Apr 22;23(8):731-6. doi: 10.1016/j.cub.2013.03.039. 
            Epub 2013 Apr 4.
  PUBMED    23562268
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2013.03.039"; journalName: "Curr Biol"; date: 
            "2013-04-22- 22"; volume: "23"; issue: "8"; pages: "731-6"
COMMENT     Sequence Label: mEmerald-MyosinIIC-N-18
FEATURES             Location/Qualifiers
     source          1..6822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6071..6080
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6077..6079,6080..6082,6083..6796)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6122..6143)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6383..6402)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6730..6751
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagatccgct agcaccgcca ccatggctgc agtgaccatg tccgtgtctg ggaggaaggt
       61 agcctccagg ccaggcccgg tgcctgaggc agcccaatcg ttcctctacg cgccccggac
      121 gccaaatgta ggtggccctg gagggccaca ggtggagtgg acagcccggc gcatggtgtg
      181 ggtgccctcg gaactgcatg ggttcgaggc agcagccctg cgggatgaag gggaggagga
      241 ggcagaagtg gagctggcgg agagtgggcg ccgcctgcgg ctgcccaggg accagatcca
      301 gcgcatgaac ccacccaagt tcagcaaggc agaagatatg gctgagctca cctgcctcaa
      361 cgaggcctcg gtcctgcaca acctgcgaga acgctactac tccgggctca tttataccta
      421 ctctggcctc ttctgtgtgg tcattaaccc atacaagcag ctgcccatct acacggaggc
      481 cattgttgaa atgtaccggg gcaagaagcg ccatgaggtg ccacctcacg tgtatgctgt
      541 gacggagggc gcgtaccgca gcatgcttca ggatcgtgag gatcaatcca ttctctgcac
      601 gggagagtct ggcgctggga agacggagaa caccaagaag gtcatccagt acctggccca
      661 tgtggcatca tctccaaagg gcaggaagga gcctggtgtc cctgcctccg tcagcaccat
      721 gtcttatggg gagctagagc gtcagcttct tcaagccaac cccatcctag aggcctttgg
      781 caatgccaag acagtgaaga acgacaactc ttcccgattt ggcaaattca tccgcatcaa
      841 ctttgatatt gctggctaca tcgtgggagc aaacatcgag acctatctgt tggagaagtc
      901 ccgggccatc agacaggcca aggatgaatg cagcttccat atcttctacc agctgctagg
      961 gggcgctggg gagcagctaa aagctgacct ccttctggag ccctgttccc attatcgctt
     1021 cctgaccaat gggccctcat cgtccccggg ccaggagcgt gagttattcc aggagaccct
     1081 ggagtccctg cgtgtgctgg gcctcctccc agaagagatc actgccatgc tgcgcactgt
     1141 ctctgctgtc ctccagtttg gcaacattgt cctgaagaaa gagcgcaata cggaccaagc
     1201 caccatgcct gacaacacag ctgcccagaa gctttgccgc ctcttgggac tcggagtgac
     1261 cgacttctcc agagcccttc tcacaccccg catcaaagtg ggccgagatt atgttcagaa
     1321 agcacaaacc aaggagcagg ctgactttgc gctggaggct ctggccaaag ctacctatga
     1381 gcgcctgttc cgctggctgg ttctgcggct caaccgtgcc ctggacagaa gcccgcggca
     1441 gggtgcctcc ttcctgggca tcctggacat cgcgggcttt gagatcttcc agctgaactc
     1501 cttcgagcag ctgtgcatca actacaccaa cgagaagcta cagcagctat tcaaccacac
     1561 catgttcgtg ctggagcagg aggagtacca gcgagagggc atcccctgga ccttcctaga
     1621 cttcgggttg gacctgcaac cttgcatcga cctcattgag cgtccggcca accctccagg
     1681 tctcctggcc ctgctggacg aggagtgctg gttccccaag gccacggaca agtcttttgt
     1741 ggagaaggtc gcccaggagc agggcagcca ccccaaattc cagcgcccca ggaacctgcg
     1801 agatcaggcc gacttcagcg tcctgcacta cgccggcaag gttgactaca aagccagtga
     1861 gtggctgatg aagaacatgg acccactgaa tgacaatgtg gccgccttgc ttcaccagag
     1921 cacggatcgt ctcacagctg agatctggaa ggatgtggag ggcatcgtgg ggctggagca
     1981 agtaagcagc cttggagatg gcccaccggg aggccgcccc cgccgtggaa tgtttcggac
     2041 tgtggggcag ctctacaaag aatccctgag ccgcctcatg gccacgctca gcaacaccaa
     2101 ccctagtttt gtccgctgca tcgttcccaa tcatgagaag agggctggaa agctggagcc
     2161 gcgcctggtg ctggaccaac tccgttgtaa cggggtcctc gagggtatac gcatctgtcg
     2221 ccaaggcttc cccaaccgca tcctcttcca ggagttccga cagcgctatg aaatcctcac
     2281 cccgaacgct attcccaagg gcttcatgga cggcaaacag gcctgtgaga agatgatcca
     2341 ggccctggag ctagacccca acctgtaccg tgttggccaa agcaagatct tcttccgggc
     2401 aggggtcctg gcccagctgg aggaggagcg ggacctgaaa gtcaccgaca tcatagtgtc
     2461 tttccaggca gcggcacggg gctacctggc ccgtagggct ttccagagac ggcagcagca
     2521 gcagagtgct ctgagggtga tgcagagaaa ctgtgctgcc tacctcaagc tcaggaactg
     2581 gcagtggtgg aggctgttca tcaaggtgaa gcccctgctg caggtgacac ggcaggatga
     2641 ggtgctgcag gcgcgcgccc aggagctgca gaaagttcag gagctgcagc agcagagcgc
     2701 tcgtgaagtg ggggaactgc agggtcgagt ggcacagcta gaggaggagc gcacgcgcct
     2761 ggctgagcag cttcgagcag aagccgagct ctgctctgag gccgaggaga cgcgggcgcg
     2821 actggctgcc cggaagcagg agctggagct ggtggtgaca gagctggagg cacgagtggg
     2881 cgaggaagaa gagtgcagcc ggcagctgca gagtgagaag aagaggctgc agcagcatat
     2941 ccaggagcta gagagccacc tggaagctga ggagggtgcc cggcagaagc tacagctgga
     3001 gaaggtgacc acagaggcca agatgaagaa atttgaggag gacctgctgc tcctggagga
     3061 ccagaattcc aagctgagca aggagcggag gctgctggag gagcggctgg ctgagttctc
     3121 ctcacaggca gcagaagagg aagagaaagt caaaagtctc aacaagctga ggctcaaata
     3181 tgaagccaca atctcagaca tggaagaccg gctgaagaag gaggagaagg gacgccagga
     3241 actagagaag ctgaagcgac ggctggacgg ggagagctca gagcttcagg agcagatggt
     3301 ggagcagaag cagagggcag aggaactgct cgcacagctg ggccgcaagg aggatgagct
     3361 gcaggccgcc ctgctcaggg cagaggaaga gggtggtgcc cgtgcccagt tgctcaagtc
     3421 cctgcgagag gcacaggctg gccttgctga ggctcaggag gacctggaag ctgagcgggt
     3481 agccagggcc aaggcggaga agcagcgccg ggacctgggc gaggagttgg aggccctacg
     3541 tggggagctc gaggacactc tggattccac caacgcccag caggagctgc ggtccaagag
     3601 ggagcaggag gtgacagagc tgaagaaagc attggaagag gagtcccgtg cccatgaggt
     3661 gtccatgcag gagctgagac agaggcatag ccaggcactg gtggagatgg ccgagcagtt
     3721 ggagcaagcc cggaggggca aaggtgtgtg ggagaagact cggctatccc tggaggctga
     3781 ggtgtccgag ctgaaggccg agctgagcag cctgcagacc tcgagacagg agggtgagca
     3841 gaagaggcgc cgcctggagt cccagctaca ggaggtccag ggccgatcca gtgattcgga
     3901 gcgggctcgg tctgaggctg ctgagaagct gcagagagcc caggcggaac ttgagagcgt
     3961 gtccacagcc ctgagtgagg cggagtccaa agccatcagg ctgggcaagg agctgagcag
     4021 tgcagagtcc cagctgcatg acacccagga actgcttcag gaggagacca gggcaaagct
     4081 ggccttgggg tcccgtgtgc gtgccctaga ggccgaggcg gcggggcttc gggagcagat
     4141 ggaagaggag gtggttgcca gggaacgggc tggccgggag ctgcagagca cgcaggccca
     4201 gctctctgaa tggcggcgcc gccaggaaga agaggctgcg gtgctggagg ctggggagga
     4261 ggctcggcgc cgtgcagccc gggaggcaga gaccctgacc cagcgcctgg cagaaaagac
     4321 tgaggctgta gaacgactgg agcgagcccg gcgccgactg cagcaggagt tggacgatgc
     4381 cactgtggat ctggggcagc agaagcagct cctgagcaca ctggagaaga agcagcggaa
     4441 atttgaccag ctcctggcag aggagaaggc tgcagttcta cgggctgtgg aagaccgtga
     4501 acggatagag gccgaaggcc gggagcgaga ggcccgggcc ctgtcgctga cccgggccct
     4561 ggaagaggag caggaggccc gggaggagct ggagaggcag aaccgtgctc tgagggctga
     4621 gctggaagca ctgctgagca gcaaggatga cgtgggcaag aacgtgcacg agctggagcg
     4681 agcccgtaag gcggctgaac aggcagccag tgacctgcgg acacaggtga cagaattgga
     4741 ggatgagctg acagccgcag aggatgccaa gctgcgcctg gaggtgactg tgcaggctct
     4801 gaaggctcaa catgaacgcg acctgcaggg ccgcgatgat gccggtgagg agaggcggag
     4861 gcagctggcc aagcagctaa gagacgcaga ggtagagcgc gatgaggaac ggaagcagag
     4921 ggcactggct atggctgccc gcaagaagct ggagctggaa ctggaggagt tgaaggcgca
     4981 gacatctgct gctgggcagg gcaaggaaga ggcagtgaag cagctgaaga agatgcaggt
     5041 ccagatgaag gagctgtggc gggaggtaga ggagacgcgt agctcccgcg acgagatgtt
     5101 taccctgagc agggaaaatg agaagaagct caaggggctg gaagctgagg tgctgcgtct
     5161 gcaagaggaa cttgctgcct cagaccgagc ccggaggcag gcccagcaag acagagacga
     5221 gatggcagag gaggtggcca gtggcaatct tagcaaggca gccaccctgg aggaaaaacg
     5281 gcagctggag gggcgactga gccagttgga agaggagctg gaggaagaac agaacaactc
     5341 ggagctgctc aaggaccatt accgaaagct agtgctacag gtcgagtccc tcaccacaga
     5401 actgtctgcc gaacgaagtt tctcagccaa ggccgagagt ggacggcagc agctggagcg
     5461 gcagatccag gaactgcggg cccgcttggg tgaagaggat gctggagccc gagccaggca
     5521 gaaaatgctg atcgctgctc tggagtctaa actggcccag gcagaggagc agctggagca
     5581 ggagagcagg gagcgcatcc tctctggcaa gctggtacgc agagctgaga agcggctgaa
     5641 ggaggtagtt cttcaggtgg atgaagagcg cagggtggct gaccaggtcc gggaccagct
     5701 ggagaaaagc aacctccggc tgaagcagct caagaggcag ctggaggagg cagaggagga
     5761 ggcatctcgg gcacaggctg gtcggaggcg gctgcagcgg gagctggagg acgtcactga
     5821 gtctgcagaa tccatgaacc gggaggtgac cacgctgagg aacaggctcc ggcgtggccc
     5881 acttacattc accacacgga ctgtgcgcca ggtgttccgg ctggaagagg gcgtggcttc
     5941 tgacgaggaa gaggctgaag gagctgaacc tggctctgca ccaggccagg agccggaggc
     6001 tccgccccct gccacacccc aaagcagcgg tggagcaagc gcagccagtg gtagcgcgga
     6061 tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     6121 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     6181 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     6241 cgtgccctgg cccaccctcg tgaccacctt gacctacggc gtgcagtgct tcgcccgcta
     6301 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     6361 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     6421 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     6481 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaaggtct atatcaccgc
     6541 cgacaagcag aagaacggca tcaaggtgaa cttcaagacc cgccacaaca tcgaggacgg
     6601 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     6661 gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa
     6721 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     6781 cgagctgtac aagtaagcgg ccgcgactct agatcataat ca
//
