LOCUS       sequence-84345-e        2324 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2324)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Myotilin-C-14
FEATURES             Location/Qualifiers
     source          1..2324
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     681..702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc
      241 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagtccgga agtgctggga gcgcaagcgg aagcggaggc
      781 tccggtttta actacgaacg tccaaaacac ttcatccagt cccaaaaccc atgtggctcc
      841 agattgcagc ctcctggacc agaaacctcc agcttctcta gccagaccaa acagtcttcc
      901 attatcatcc agccccgcca gtgtacagag caaagatttt ctgcctcctc aacactgagc
      961 tctcacatca ccatgtcctc ctctgctttc cctgcttctc cccagcagca tgctggctcc
     1021 aacccaggcc aaagggttac aaccacctat aaccagtccc cagccagctt cctcagctcc
     1081 atattaccat cacagcctga ttacaatagc agtaaaatcc cttccgctat ggattccaac
     1141 tatcaacagt cctcagctgg ccaacctata aatgcaaagc catcccaaac tgcaaatgct
     1201 aagcccatac caagaactcc tgatcatgaa atacaaggat caaaagaagc tttgattcaa
     1261 gatttggaaa gaaagctgaa atgcaaggac acccttcttc ataatggaaa tcaacgtcta
     1321 acatatgaag agaagatggc tcgcagattg ctaggaccac agaatgcagc tgctgtgttt
     1381 caagctcagg atgacagtgg tgcacaagac tcgcagcaac acaactcaga acatgcgcga
     1441 ctgcaagttc ctacatcaca agtaagaagt agatcaacct caaggggaga tgtgaatgat
     1501 caggatgcaa tccaggagaa attttaccca ccacgtttca ttcaagtgcc agagaacatg
     1561 tcgattgatg aaggaagatt ctgcagaatg gacttcaaag tgagtggact gccagctcct
     1621 gatgtgtcat ggtatctaaa tggaagaaca gttcaatcag atgatttgca caaaatgata
     1681 gtgtctgaga agggtcttca ttcactcatc tttgaagtag tcagagcttc agatgcaggg
     1741 gcttatgcat gtgttgccaa gaatagagca ggagaagcca ccttcactgt gcagctggat
     1801 gtccttgcaa aagaacataa aagagcacca atgtttatct acaaaccaca gagcaaaaaa
     1861 gttttagagg gagattcagt gaaactagaa tgccagatct cggctatacc tccaccaaag
     1921 cttttctgga aaagaaataa tgaaatggta caattcaaca ctgaccgaat aagcttatat
     1981 caagataaca ctggaagagt tactttactg ataaaagatg taaacaagaa agatgctggg
     2041 tggtatactg tgtcagcagt taatgaagct ggagtgacta catgtaacac aagattagac
     2101 gttacggcac gtccaaacca aactcttcca gctcctaagc agttacgggt tcgaccaaca
     2161 ttcagcaaat atttagcact taatgggaaa ggtttgaatg taaaacaagc ttttaaccca
     2221 gaaggagaat ttcagcgttt ggcagctcaa tctggactct atgaaagtga agaactttaa
     2281 ggatccaccg gatctagata actgatcata atcagccata ccac
//
