LOCUS       sequence-84346-e        2334 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2334)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Myotilin-N-14
FEATURES             Location/Qualifiers
     source          1..2334
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1569..1578
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1575..1577,1578..1580,1581..2294)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1620..1641)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1881..1900)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2228..2249
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atccgctagc gctaccggac tcagatctcg aggccaccat gtttaactac gaacgtccaa
       61 aacacttcat ccagtcccaa aacccatgtg gctccagatt gcagcctcct ggaccagaaa
      121 cctccagctt ctctagccag accaaacagt cttccattat catccagccc cgccagtgta
      181 cagagcaaag attttctgcc tcctcaacac tgagctctca catcaccatg tcctcctctg
      241 ctttccctgc ttctccccag cagcatgctg gctccaaccc aggccaaagg gttacaacca
      301 cctataacca gtccccagcc agcttcctca gctccatatt accatcacag cctgattaca
      361 atagcagtaa aatcccttcc gctatggatt ccaactatca acagtcctca gctggccaac
      421 ctataaatgc aaagccatcc caaactgcaa atgctaagcc cataccaaga actcctgatc
      481 atgaaataca aggatcaaaa gaagctttga ttcaagattt ggaaagaaag ctgaaatgca
      541 aggacaccct tcttcataat ggaaatcaac gtctaacata tgaagagaag atggctcgca
      601 gattgctagg accacagaat gcagctgctg tgtttcaagc tcaggatgac agtggtgcac
      661 aagactcgca gcaacacaac tcagaacatg cgcgactgca agttcctaca tcacaagtaa
      721 gaagtagatc aacctcaagg ggagatgtga atgatcagga tgcaatccag gagaaatttt
      781 acccaccacg tttcattcaa gtgccagaga acatgtcgat tgatgaagga agattctgca
      841 gaatggactt caaagtgagt ggactgccag ctcctgatgt gtcatggtat ctaaatggaa
      901 gaacagttca atcagatgat ttgcacaaaa tgatagtgtc tgagaagggt cttcattcac
      961 tcatctttga agtagtcaga gcttcagatg caggggctta tgcatgtgtt gccaagaata
     1021 gagcaggaga agccaccttc actgtgcagc tggatgtcct tgcaaaagaa cataaaagag
     1081 caccaatgtt tatctacaaa ccacagagca aaaaagtttt agagggagat tcagtgaaac
     1141 tagaatgcca gatctcggct atacctccac caaagctttt ctggaaaaga aataatgaaa
     1201 tggtacaatt caacactgac cgaataagct tatatcaaga taacactgga agagttactt
     1261 tactgataaa agatgtaaac aagaaagatg ctgggtggta tactgtgtca gcagttaatg
     1321 aagctggagt gactacatgt aacacaagat tagacgttac ggcacgtcca aaccaaactc
     1381 ttccagctcc taagcagtta cgggttcgac caacattcag caaatattta gcacttaatg
     1441 ggaaaggttt gaatgtaaaa caagctttta acccagaagg agaatttcag cgtttggcag
     1501 ctcaatctgg actctatgaa agtgaagaac ttggcggaag cggcgggtcc ggaggggatc
     1561 caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     1621 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1681 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1741 tgccctggcc caccctcgtg accaccttga cctacggcgt gcagtgcttc gcccgctacc
     1801 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1861 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1921 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1981 acatcctggg gcacaagctg gagtacaact acaacagcca caaggtctat atcaccgccg
     2041 acaagcagaa gaacggcatc aaggtgaact tcaagacccg ccacaacatc gaggacggca
     2101 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     2161 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
     2221 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     2281 agctgtacaa gtaagcggcc gcgactctag atcataatca gccataccac attt
//
