LOCUS       sequence-84347-e        2384 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton-Signalling , Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2384)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-N-Wasp-C-18
FEATURES             Location/Qualifiers
     source          1..2384
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(45..47,48..50,51..761)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(90..111)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(351..370)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     698..719
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccaagctagt gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg
       61 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      121 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      181 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga
      241 cctacggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
      301 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      361 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      421 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      481 acaacagcca caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact
      541 tcaagacccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
      601 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
      661 ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
      721 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggaagc ggcggttccg
      781 ggagcggaag cggctctgga tcaggcagcg gagggagctc gggccagcag cccccgcgga
      841 gggtcaccaa cgtgggctcc ctgctgctca ccccgcaaga gaacgagtct ctcttctcct
      901 tcctcggcaa gaaatgtgtg actatgtctt cagcagtggt gcagttgtat gcagcagatc
      961 ggaactgtat gtgggcaaag aagtgcagtg gtgtcgcttg tctggttaag gacaatcctc
     1021 agagatcttt ttttttaaga atatttgaca ttaaggatgg gaaattactg tgggaacaag
     1081 agctatacaa taactttgta tataatagtc ctagaggata ttttcatacc tttgctggag
     1141 atacttgtca agtagctctt aattttgcca atgaagaaga agcaaaaaag ttccgaaaag
     1201 cagttacaga cctgttgggc cgacgacaaa ggaaatctga aaaaagacga gatgctccaa
     1261 atggtcccaa tctacccatg gctacagttg acataaaaaa tccagaaatc acaacaaata
     1321 gattttatgg ttcacaagtc aacaacatct cccacaccaa agaaaagaag aaaggaaaag
     1381 ctaaaaagaa gagattaacc aaggcagata ttggaacacc aagtaatttc cagcacattg
     1441 ggcatgttgg atgggatcca aatacaggtt ttgatctaaa taatttggat ccagaattaa
     1501 agaatctttt tgatatgtgt gggatctctg aggcccagct taaagacaga gaaacatcaa
     1561 aagttattta tgactttatt gaaaaaacag gaggtgtaga agctgttaaa aacgaactcc
     1621 gaagacaagc accaccacca cctccaccct cgagaggagg acctccccct cctcctcccc
     1681 ctcctcatag ctcaggtcct cctccccctc ctgcccgagg aaggggggct cctcctcccc
     1741 caccttcaag agctcctact gctgcacctc cacctccacc tccttctagg cctggtgttg
     1801 ttgttcctcc gccccctcca aacaggatgt accctcctcc accaccagcg ctgccctctt
     1861 cagcaccttc aggccctcca ccacctccac ctccatctat ggcagggtcc acagcaccac
     1921 cacctcctcc tccacctccg cccccaccag ggccaccacc tccccctggc ctgccttctg
     1981 atggtgacca tcaagttcca gctccttcag gaaacaaagc agctcttttg gatcaaatta
     2041 gagagggtgc tcagctgaaa aaagtggaac agaatagccg gcccgtgtcc tgctcaggaa
     2101 gggatgcgct tttagaccag atacgacagg gcattcaatt gaaatctgtg tctgatggcc
     2161 aagagtccac accaccaaca cccgcaccca cttcaggaat tgtgggtgcg ctgatggaag
     2221 tgatgcagaa aaggagcaaa gccattcatt cctcagatga agatgaagac gatgatgatg
     2281 aagaagattt tgaggatgat gatgagtggg aagactgaga attctgcagt cgacggtacc
     2341 gcgggcccgg gatccaccgg atctagataa ctgatcataa tcag
//
