LOCUS       sequence-84349-e        1384 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endosomes, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1384)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-NEEP21-C-18
FEATURES             Location/Qualifiers
     source          1..1384
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(33..35,36..38,39..749)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(78..99)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(339..358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     686..707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(885..896)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(906..917)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca
       61 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      121 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      181 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttgacc tacggcgtgc
      241 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      301 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      361 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      421 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      481 aggtctatat caccgccgac aagcagaaga acggcatcaa ggtgaacttc aagacccgcc
      541 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      601 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
      661 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      721 tcactctcgg catggacgag ctgtacaagt ccggactcag atctcgagct caagcttcga
      781 attcagggtc tggaagtggt agcgtgaagt tggggaataa tttcgcagag aagggcacca
      841 agcagccact gctggaggat ggcttcgaca ccattccttt gatgacgccc ctcgatgtca
      901 accagctgca gttcccaccc ccagataagg tcgtggtgaa aactaagact gaatatgaac
      961 ctgatcgcaa aaaaggaaaa gcacgtcctc ccaagatagc cgagttcacc gtcagcatca
     1021 ccgagggtgt caccgagagg tttaaggtct ccgtgctggt cctctttgcc ctggccttcc
     1081 tcacctgtgt cgtcttcctg gttgtctaca aagtgtacaa gtatgaccgc gcctgccctg
     1141 gtgggtttgt cttgaagaac acccagtgca tcccagaagg cttggagagc tactacacgg
     1201 agcaagactc cagtgcccgg gagaaatttt acactgtcat aaaccactac aacgtggcca
     1261 agcagagcat cacccgctcc gtgtcgccat ggatgtcagt tctgtcagaa gagaagctgt
     1321 cggaacagga gaccgaagct gcagagaagt cagcctaggg atccaccgga tctagataac
     1381 tgat
//
