LOCUS       sequence-84351-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Nucleus-7
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        23..74
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     25..45
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(119..121,122..124,125..835)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(164..185)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(425..444)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     772..793
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             860..880
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             884..904
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             908..928
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
       61 atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat
      121 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      181 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
      241 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
      301 cgtgaccacc ttgacctacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
      361 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
      421 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      481 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
      541 gctggagtac aactacaaca gccacaaggt ctatatcacc gccgacaagc agaagaacgg
      601 catcaaggtg aacttcaaga cccgccacaa catcgaggac ggcagcgtgc agctcgccga
      661 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
      721 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
      781 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg
      841 actcagatct cgagctgatc caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa
      901 ggtagatcca aaaaagaaga gaaaggtagg atccaccgga tctagataac tgatcataat
      961 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     1021 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtt
//
