LOCUS       sequence-84352-e        1846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Pore, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1846)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Nup35-C-18
FEATURES             Location/Qualifiers
     source          1..1846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctggca gcggtggagg
      781 cagcgcatcc ggcggaagcg gaagcgcagc ctttgcagtg gaacctcagg ggcccgcgtt
      841 aggatctgaa ccaatgatgc tgggttcacc cacatctcca aagccaggag ttaatgccca
      901 gttcttacct ggatttttaa tgggggattt gccagctccg gtgactccac aacctcgatc
      961 aattagtggc ccttcagtag gagtaatgga aatgagatca cctttacttg caggtgggtc
     1021 accaccacaa ccagttgtac cagctcataa agataaaagt ggcgctccac cagttagaag
     1081 tatatatgat gacatttcta gcccaggact tggatcaaca cctttaactt caagaagaca
     1141 gccaaacatt tcagtaatgc agagtcctct tgttggagtt acatctactc ctggaacagg
     1201 gcaaagtatg tttagtccag caagtatcgg tcagccacga aagacgacat tatctcctgc
     1261 ccagttggat cctttttata ctcaaggaga ttctttgact tcagaagatc acctcgatga
     1321 ctcttgggtg actgtatttg ggtttcctca agcatctgct tcctacatat tactacaatt
     1381 tgcacagtat gggaatatct taaaacatgt gatgtctaat acaggaaatt ggatgcatat
     1441 tcgttatcaa tctaaactgc aggctcggaa agccttaagc aaagatggga ggatttttgg
     1501 agaatccatc atgattggtg taaaaccatg tattgacaaa agtgttatgg aaagcagtga
     1561 cagatgtgct ttatcatctc catctttagc ctttacacca ccaatcaaaa ctctaggtac
     1621 accaacacaa cctggaagta ctcctaggat ttctaccatg agacctcttg ctacagcata
     1681 caaagcctct actagtgatt atcaggttat ttctgacaga caaacgccaa aaaaagatga
     1741 aagtcttgta tccaaagcaa tggagtacat gtttggctgg taggaattct gcagtcgacg
     1801 gtaccgcggg cccgggatcc accggatcta gataactgat cataat
//
