LOCUS       sequence-84353-e        1846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Pore, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1846)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Nup35-N-14
FEATURES             Location/Qualifiers
     source          1..1846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1072..1081
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1078..1080,1081..1083,1084..1797)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1123..1144)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1384..1403)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1731..1752
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acatgctagt gaccgtcaga tccgctagcg ctaccggact cagatctcga gaccgccatg
       61 gcagcctttg cagtggaacc tcaggggccc gcgttaggat ctgaaccaat gatgctgggt
      121 tcacccacat ctccaaagcc aggagttaat gcccagttct tacctggatt tttaatgggg
      181 gatttgccag ctccggtgac tccacaacct cgatcaatta gtggcccttc agtaggagta
      241 atggaaatga gatcaccttt acttgcaggt gggtcaccac cacaaccagt tgtaccagct
      301 cataaagata aaagtggcgc tccaccagtt agaagtatat atgatgacat ttctagccca
      361 ggacttggat caacaccttt aacttcaaga agacagccaa acatttcagt aatgcagagt
      421 cctcttgttg gagttacatc tactcctgga acagggcaaa gtatgtttag tccagcaagt
      481 atcggtcagc cacgaaagac gacattatct cctgcccagt tggatccttt ttatactcaa
      541 ggagattctt tgacttcaga agatcacctc gatgactctt gggtgactgt atttgggttt
      601 cctcaagcat ctgcttccta catattacta caatttgcac agtatgggaa tatcttaaaa
      661 catgtgatgt ctaatacagg aaattggatg catattcgtt atcaatctaa actgcaggct
      721 cggaaagcct taagcaaaga tgggaggatt tttggagaat ccatcatgat tggtgtaaaa
      781 ccatgtattg acaaaagtgt tatggaaagc agtgacagat gtgctttatc atctccatct
      841 ttagccttta caccaccaat caaaactcta ggtacaccaa cacaacctgg aagtactcct
      901 aggatttcta ccatgagacc tcttgctaca gcatacaaag cctctactag tgattatcag
      961 gttatttctg acagacaaac gccaaaaaaa gatgaaagtc ttgtatccaa agcaatggag
     1021 tacatgtttg gctggggggg tgcgtcaggc ggcagcggat ctggaccggt cgccaccatg
     1081 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1141 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1201 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1261 gtgaccacct tgacctacgg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
     1321 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1381 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1441 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1501 ctggagtaca actacaacag ccacaaggtc tatatcaccg ccgacaagca gaagaacggc
     1561 atcaaggtga acttcaagac ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     1621 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1681 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1741 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagcg
     1801 gccgcgactc tagatcataa tcagccatac cacattgtag agtttt
//
