LOCUS       sequence-84354-e        2201 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Pore, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2201)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Nup50-C-10
FEATURES             Location/Qualifiers
     source          1..2201
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(19..21,22..24,25..735)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(64..85)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(325..344)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     672..693
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
       61 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      121 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      181 cccgtgccct ggcccaccct cgtgaccacc ttgacctacg gcgtgcagtg cttcgcccgc
      241 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      301 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      361 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      421 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaaggt ctatatcacc
      481 gccgacaagc agaagaacgg catcaaggtg aacttcaaga cccgccacaa catcgaggac
      541 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      601 ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag
      661 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      721 gacgagctgt acaagtccgg actcagatct ggcagcagtg gaggtgccaa aagaaatgcc
      781 gagaaggaac tgacagatag gaattgggat caagaagatg aagctgaaga ggtgggaaca
      841 ttctccatgg ccagtgagga agtcttgaag aatagagcca taaagaaagc aaagcgtaga
      901 aatgttggat ttgaatctga cactggagga gcctttaaag gttttaaagg tttggtggta
      961 ccttctggag gaggacgctt ttctggattt ggtagtggcg ctggagggaa gcctttggaa
     1021 ggactgtcga atggaaacaa cataaccagt gcccctccct tcgccagtgc aaaggcagcg
     1081 gcagatccca aggtagcctt tggttctctt gctgcaaatg gccctaccac cttggttgat
     1141 aaagtttcaa atcccaaaac taatggggac agtcagcagc cctcctcctc tggccttgct
     1201 tccagtaaag cttgtgtcgg aaatgcctat cacaagcagt tggccgcctt gaactgctcc
     1261 gtgcgggatt ggatagtgaa gcacgtgaat acaaaccccc tctgtgatct gacacctatc
     1321 tttaaagact atgagaaata tttagcaaac attgaacagc aacacgggaa cagtggcagg
     1381 aattctgaaa gtgaatctaa caaagtggca gctgaaacac agtctccttc cctttttggc
     1441 tcaacaaaat tacagcaaga gtcaacgttt ttgtttcatg gcaacaaaac tgaagataca
     1501 cctgacaaga agatggaggt ggcatctgaa aagaaaacgg acccatcatc actaggagcg
     1561 acaagtgcct catttaattt cggcaagaaa gttgatagct ctgttttggg ctcattaagc
     1621 tctgtccccc tgactggatt ttctttctcc cctggaaact ccagtttatt tggcaaagat
     1681 actacccaga gtaaaccagt ctcttcacca tttcccacta aaccattgga gggccaagca
     1741 gaaggtgaca gtggtgaatg caaaggtgga gatgaagaag agaatgatga gccacccaaa
     1801 gtagtagtta ccgaagtaaa agaagaagac gctttttact ccaaaaagtg taaactgttt
     1861 tacaagaaag acaatgagtt taaagagaaa ggcataggta ctctgcattt aaaacctaca
     1921 gcaaatcaga agacacagct tttggtgcgg gcagacacca atttaggcaa catattgctg
     1981 aacgttctga ttccacccaa tatgccatgt acgcgaacag ggaagaataa cgttcttatc
     2041 gtctgtgttc caaatccacc aattgacgag aagaatgcca ccatgccagt caccatgttg
     2101 attcgggtaa aaaccagcga ggatgcagac gagttgcaca aaattttact ggagaaaaag
     2161 gatgcctgag gatccaccgg atctaaccgg atctagataa c
//
