LOCUS       sequence-84355-e        2201 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Pore, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2201)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Nup50-N-10
FEATURES             Location/Qualifiers
     source          1..2201
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1457..1466
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1463..1465,1466..1468,1469..2182)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1508..1529)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1769..1788)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2116..2137
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacggtcaga tccgctagcg atgccaccat ggccaaaaga aatgccgaga aggaactgac
       61 agataggaat tgggatcaag aagatgaagc tgaagaggtg ggaacattct ccatggccag
      121 tgaggaagtc ttgaagaata gagccataaa gaaagcaaag cgcagaaatg ttggatttga
      181 atctgacact ggaggagcct ttaaaggttt taaaggtttg gtggtacctt ctggaggagg
      241 acgcttttct ggatttggta gtggcgctgg agggaagcct ctggaaggac tgtcgaatgg
      301 aaacaacata accagtgccc ctcccttcgc cagtgcaaag gcagcggcag atcccaaggt
      361 agcctttggt tctcttgctg caaatggccc taccaccttg gttgataaag tttcaaatcc
      421 caaaactaat ggggacagtc agcagccctc ctcctctggc cttgcttcca gtaaagcttg
      481 tgtcggaaat gcctatcaca agcagttggc cgccttgaac tgctccgtgc gggattggat
      541 agtgaagcac gtgaatacaa accccctctg tgatctgaca cctatcttta aagactatga
      601 gaaatattta gcaaacattg aacagcaaca cgggaacagt ggcaggaatt ctgaaagtga
      661 atctaacaaa gtggcagctg aaacacagtc tccttccctt tttggctcaa caaaattaca
      721 gcaagagtca acgtttttgt ttcatggcaa caaaactgaa gatacacctg acaagaagat
      781 ggaggtggca tctgaaaaga aaacggaccc atcatcacta ggagcgacaa gtgcctcatt
      841 taatttcggc aagaaagttg atagctctgt tttgggctca ttaagctctg tccccctgac
      901 tggattttct ttctcccctg gaaactccag tttatttggc aaagatacta cccagagtaa
      961 accagtctct tcaccatttc ccactaaacc attggagggc caagcagaag gtgacagtgg
     1021 tgaatgcaaa ggtggagatg aagaagagaa tgatgagcca cccaaagtag tagttaccga
     1081 agtaaaagaa gaagacgctt tttactccaa aaagtgtaaa ctgttttaca agaaagacaa
     1141 tgagtttaaa gagaaaggca taggtactct gcatttaaaa cctacagcaa atcagaagac
     1201 acagcttttg gtgcgggcag acaccaattt aggcaacata ttgctgaacg ttctgattcc
     1261 acccaatatg ccatgtacgc gaacagggaa gaataacgtt cttatcgtct gtgttccaaa
     1321 tccaccaatt gacgagaaga atgccaccat gccagtcacc atgttgattc gggtaaaaac
     1381 cagcgaggat gcagacgagt tgcacaaaat tttactggag aaaaaggatg ccagctccgg
     1441 aggggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     1501 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1561 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1621 gctgcccgtg ccctggccca ccctcgtgac caccttgacc tacggcgtgc agtgcttcgc
     1681 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1741 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1801 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1861 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca aggtctatat
     1921 caccgccgac aagcagaaga acggcatcaa ggtgaacttc aagacccgcc acaacatcga
     1981 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     2041 cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa
     2101 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2161 catggacgag ctgtacaagt aagcggccgc gactctagat c
//
