LOCUS       sequence-84356-e        2414 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2414)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Occludin-C-14
FEATURES             Location/Qualifiers
     source          1..2414
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(29..31,32..34,35..745)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(74..95)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(335..354)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     682..703
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     RBS             811..819
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
       61 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      121 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      181 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttgacctacg gcgtgcagtg
      241 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      301 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      361 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      421 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaaggt
      481 ctatatcacc gccgacaagc agaagaacgg catcaaggtg aacttcaaga cccgccacaa
      541 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      601 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga
      661 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      721 tctcggcatg gacgagctgt acaagtccgg actcagatct cgaggaagcg gcggatctgg
      781 gtccggatca tccaggcctc ttgaaagtcc acctccttac aggcctgatg aattcaaacc
      841 gaatcattat gcaccaagca atgacatata tggtggagag atgcatgttc gaccaatgct
      901 ctctcagcca gcctactctt tttacccaga agatgaaatt cttcacttct acaaatggac
      961 ctctcctcca ggagtgattc ggatcctgtc tatgctcatt attgtgatgt gcattgccat
     1021 ctttgcctgt gtggcctcca cgcttgcctg ggacagaggc tatggaactt cccttttagg
     1081 aggtagtgta ggctaccctt atggaggaag tggctttggt agctacggaa gtggctatgg
     1141 ctatggctat ggttatggct atggctacgg aggctataca gacccaagag cagcaaaggg
     1201 cttcatgttg gccatggctg ccttttgttt cattgccgcg ttggtgatct ttgttaccag
     1261 tgttataaga tctgaaatgt ccagaacaag aagatactac ttaagtgtga taatagtgag
     1321 tgctatcctg ggcatcatgg tgtttattgc cacaattgtc tatataatgg gagtgaaccc
     1381 aactgctcag tcttctggat ctctatatgg ttcacaaata tatgccctct gcaaccaatt
     1441 ttatacacct gcagctactg gactctacgt ggatcagtat ttgtatcact actgtgttgt
     1501 ggatccccag gaggccattg ccattgtact ggggttcatg attattgtgg cttttgcttt
     1561 aataattttc tttgctgtga aaactcgaag aaagatggac aggtatgaca agtccaatat
     1621 tttgtgggac aaggaacaca tttatgatga gcagcccccc aatgtcgagg agtgggttaa
     1681 aaatgtgtct gcaggcacac aggacgtgcc ttcaccccca tctgactatg tggaaagagt
     1741 tgacagtccc atggcatact cttccaatgg caaagtgaat gacaagcggt tttatccaga
     1801 gtcttcctat aaatccacgc cggttcctga agtggttcag gagcttccat taacttcgcc
     1861 tgtggatgac ttcaggcagc ctcgttacag cagcggtggt aactttgaga caccttcaaa
     1921 aagagcacct gcaaagggaa gagcaggaag gtcaaagaga acagagcaag atcactatga
     1981 gacagactac acaactggcg gcgagtcctg tgatgagctg gaggaggact ggatcaggga
     2041 atatccacct atcacttcag atcaacaaag acaactgtac aagaggaatt ttgacactgg
     2101 cctacaggaa tacaagagct tacaatcaga acttgatgag atcaataaag aactctcccg
     2161 tttggataaa gaattggatg actatagaga agaaagtgaa gagtacatgg ctgctgctga
     2221 tgaatacaat agactgaagc aagtgaaggg atctgcagat tacaaaagta agaagaatca
     2281 ttgcaagcag ttaaagagca aattgtcaca catcaagaag atggttggag actatgatag
     2341 acagaaaaca tagggtaccg cgggcccggg atccaccgga tctagataac tgatcataat
     2401 cagccatacc acat
//
