LOCUS       sequence-84358-e        2837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2837)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Palladin-C-7
FEATURES             Location/Qualifiers
     source          1..2837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttgac ctacggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      481 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc
      541 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagt gagcgctctg
      781 gcctcccgcg ctaccgccat gcagtcctcc ggctccttca actacgcgcg ccctaagcag
      841 ttcatcgcgg cgcagaacct gggtcccgca tcggggctgc ccacgccaac ctccagcccc
      901 agctcctcca gcttgccgtc accattgtcc cccacgccca ggcccttcgg ccgcgcgccc
      961 gggccgccct tcgtggaacc cgaggccatg tggggcccgt cctcgccctc gccgccaccg
     1021 ccgccacctc cggtcttcag cccctctgca gcctacccgg tgcccgacgt gttcccactg
     1081 ccaccgccgc cgccaccgct acctagctcc acctcacact gcgcctcgcc cgcccgcttc
     1141 ggccccagcc agacgcccgc agccttcctc agcgccctgc tgccctctca gccaccgcct
     1201 gtcgctgtca acgccctggg gctgcccaag ggagtcaccc ccgcggggtt cccaaagaag
     1261 tccagtagaa ctgctagaat tgcctctgat gaggagattc aaggcacaaa ggatgctgtc
     1321 atccaagacc tggaacggaa gcttcgcttc aaggaggacc ttctgaacaa tggccaaccg
     1381 aggctaacct atgaggaaag aatggctcgc cgcctgcttg gagccgacag cgcaaacgtc
     1441 ttcaacatcc aggagccaga ggaaacggca gccaatcagg agtacaaagt ctctagctgc
     1501 gagcagaggc tgattagcga gattgaatac aggctggagc gctcccctgt ggatgagtcg
     1561 ggagacgagg tgcaggatcc agatgtgcct gtggagaacg caacagctcc cttctttgag
     1621 atgaagctga aacactacaa gatctttgag gggatgccgg tgactttcac gtgtcgagtg
     1681 gctgggaatc caaagccaaa gatctattgg tttaaagatg ggaagcagat ttctccgaag
     1741 agcgatcact acaccattca gagagacctt gatgggacct gctctctcca caccacggcc
     1801 tctaccctag acgacgatgg gaactacacc atcatggctg ccaaccctca gggtcgcgtc
     1861 agttgtacag gaaggctaat ggtacaggct gtcaaccaaa gaggccgcag tccccgctct
     1921 ccctcaggcc atcctcatgc cagaaggcct cgctctcgat cacgggacag tggagatgaa
     1981 aacgagccca ttcaggagcg attcttcaga cctcacttcc tgcaggctcc tggagacctg
     2041 accgttcagg aaggcaagct ctgcaggatg gactgcaagg tcagtgggtt accaacccca
     2101 gatctcagct ggcaactaga cggaaagccc atacgccccg acagtgctca caagatgctg
     2161 gtccgtgaga atggggtcca ctccctcatt atagagccag tcacgtcccg ggacgccggc
     2221 atctacacat gtattgccac caacagagca ggacagaact cgtttaacct ggagcttgtg
     2281 gttgctgcta aggaagcaca caaggcccct gtgtttatgg agaagctaca gaacacgggg
     2341 gttgctgatg gatacccagt gcggctagaa tgccgtgtct ccggagtgcc gccacctcag
     2401 atattttgga agaaagaaaa tgaatcgctc actcatagca ctgagcgagt aagcatgcac
     2461 caggataatc atggctacat ctgcctgctc atccagggag ccacaaagga agacgctggg
     2521 tggtacactg tgtctgccaa gaacgaagca ggcattgtgt cctgcactgc ccggctggat
     2581 gtctacaccc agtggcacca gcagccacag accaccaagc caaaaaaagt acggccctcg
     2641 gccagtcgct acgcagcact ttcggaccag ggactagaca tcaaagccgc tttccaacct
     2701 gaagccagcc catctcacct gactctgaac agcggcttgg tagaaagtga agacctgtga
     2761 gaattctgca gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat
     2821 aatcagccat accacat
//
