LOCUS       sequence-84359-e        1982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1982)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Parvin-C-14
FEATURES             Location/Qualifiers
     source          1..1982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(33..35,36..38,39..749)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(78..99)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(339..358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     686..707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca
       61 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      121 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      181 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttgacc tacggcgtgc
      241 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      301 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      361 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      421 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      481 aggtctatat caccgccgac aagcagaaga acggcatcaa ggtgaacttc aagacccgcc
      541 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      601 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
      661 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      721 tcactctcgg catggacgag ctgtacaagt ccggaagtgc tgggagcgca agcggaagcg
      781 gaggctccgg tgccacctcc ccgcagaagt cgccttctgt ccccaagtct cccactccca
      841 agtcgccccc gtcccgcaag aaagatgatt ccttcttggg gaaactcgga gggaccctgg
      901 cccggaggaa gaaagccaag gaggtgtccg agctgcagga ggagggaatg aacgccatca
      961 acctgcccct cagcccaatt ccctttgagc tggaccccga ggacacgatg ctggaggaga
     1021 atgaggtgcg aacaatggtg gatccaaact cacgcagtga ccccaagctt caagaactga
     1081 tgaaggtatt aattgactgg attaatgatg tgttggttgg agaaagaatc attgtgaaag
     1141 acctagctga agatttgtat gatggacaag tcctgcagaa gcttttcgag aaactggaga
     1201 gtgagaagct aaatgtggct gaggtcaccc agtcagagat tgctcagaag caaaaactgc
     1261 agactgtcct ggagaagatc aatgaaaccc tgaaacttcc tcccaggagc atcaagtgga
     1321 atgtggattc tgttcatgcc aagagcctgg tggccatctt acacctgctc gttgctctgt
     1381 ctcagtattt ccgcgcacca attcgactcc cagaccatgt ttccatccaa gtggttgtgg
     1441 tccagaaacg agaaggaatc ctccagtctc ggcaaatcca agaggaaata actggtaaca
     1501 cagaggctct ttccgggagg catgaacgtg atgcctttga caccttgttc gaccatgccc
     1561 cagacaagct gaatgtggtg aaaaagacac tcatcacttt cgtgaacaag cacctgaata
     1621 aactgaacct ggaggtcaca gaactggaaa cccagtttgc agatggggtg tacctggtgc
     1681 tgctcatggg gctcctggag ggctactttg tgcccctgca cagcttcttc ctgaccccgg
     1741 acagctttga acagaaggtc ttgaatgtct cctttgcctt tgagctcatg caagatggag
     1801 ggttggaaaa gccaaaaccg cggccagaag acatagtcaa ctgtgacctg aaatctacac
     1861 tacgagtgtt gtacaacctc ttcaccaagt accgtaacgt ggagtgagaa ttctgcagtc
     1921 gacggtaccg cgggcccggg atccaccgga tctagataac tgatcataat cagccatacc
     1981 ac
//
