LOCUS       sequence-84360-e        1959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1959)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Parvin-N-16
FEATURES             Location/Qualifiers
     source          1..1959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1198..1207
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1204..1206,1207..1209,1210..1923)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1249..1270)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1510..1529)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1857..1878
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagaccgcca tggccacctc cccgcagaag
       61 tcgccttctg tccccaagtc tcccactccc aagtcgcccc cgtcccgcaa gaaagatgat
      121 tccttcttgg ggaaactcgg agggaccctg gcccggagga agaaagccaa ggaggtgtcc
      181 gagctgcagg aggagggaat gaacgccatc aacctgcccc tcagcccaat tccctttgag
      241 ctggaccccg aggacacgat gctggaggag aatgaggtgc gaacaatggt ggatccaaac
      301 tcacgcagtg accccaagct tcaagaactg atgaaggtat taattgactg gattaatgat
      361 gtgttggttg gagaaagaat cattgtgaaa gacctagctg aagatttgta tgatggacaa
      421 gtcctgcaga agcttttcga gaaactggag agtgagaagc taaatgtggc tgaggtcacc
      481 cagtcagaga ttgctcagaa gcaaaaactg cagactgtcc tggagaagat caatgaaacc
      541 ctgaaacttc ctcccaggag catcaagtgg aatgtggatt ctgttcatgc caagagcctg
      601 gtggccatct tacacctgct cgttgctctg tctcagtatt tccgcgcacc aattcgactc
      661 ccagaccatg tttccatcca agtggttgtg gtccagaaac gagaaggaat cctccagtct
      721 cggcaaatcc aagaggaaat aactggtaac acagaggctc tttccgggag gcatgaacgt
      781 gatgcctttg acaccttgtt cgaccatgcc ccagacaagc tgaatgtggt gaaaaagaca
      841 ctcatcactt tcgtgaacaa gcacctgaat aaactgaacc tggaggtcac agaactggaa
      901 acccagtttg cagatggggt gtacctggtg ctgctcatgg ggctcctgga gggctacttt
      961 gtgcccctgc acagcttctt cctgaccccg gacagctttg aacagaaggt cttgaatgtc
     1021 tcctttgcct ttgagctcat gcaagatgga gggttggaaa agccaaaacc gcggccagaa
     1081 gacatagtca actgtgacct gaaatctaca ctacgagtgt tgtacaacct cttcaccaag
     1141 taccgtaacg tggaggcatc cgggggtgcg tcaggcggca gcggatctgg accggtcgcc
     1201 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     1261 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     1321 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1381 accctcgtga ccaccttgac ctacggcgtg cagtgcttcg cccgctaccc cgaccacatg
     1441 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     1501 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     1561 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     1621 cacaagctgg agtacaacta caacagccac aaggtctata tcaccgccga caagcagaag
     1681 aacggcatca aggtgaactt caagacccgc cacaacatcg aggacggcag cgtgcagctc
     1741 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1801 cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg
     1861 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     1921 taagcggccg cgactctaga tcataatcag ccataccac
//
