LOCUS       sequence-84361-e        5133 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5133)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Patched1-N-18
FEATURES             Location/Qualifiers
     source          1..5133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4378..4387
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4384..4386,4387..4389,4390..5103)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4429..4450)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4690..4709)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5037..5058
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgaacgtcag atccggctag caccgccatg gcctcggctg gtaacgccgc cggggccctg
       61 ggcaggcagg ccggcggcgg gaggcgcaga cggaccgggg gaccgcaccg cgccgcgccg
      121 gaccgggact atctgcaccg gcccagctac tgcgacgccg ccttcgctct ggagcagatt
      181 tccaagggga aggctactgg ccggaaagcg ccgctgtggc tgagagcgaa gtttcagaga
      241 ctcttattta aactgggttg ttacattcaa aagaactgcg gcaagttttt ggttgtgggt
      301 ctcctcatat ttggggcctt cgctgtggga ttaaaggcag ctaatctcga gaccaacgtg
      361 gaggagctgt gggtggaagt tggtggacga gtgagtcgag aattaaatta tacccgtcag
      421 aagataggag aagaggctat gtttaatcct caactcatga tacagactcc aaaagaagaa
      481 ggcgctaatg ttctgaccac agaggctctc ctgcaacacc tggactcagc actccaggcc
      541 agtcgtgtgc acgtctacat gtataacagg caatggaagt tggaacattt gtgctacaaa
      601 tcaggggaac ttatcacgga gacaggttac atggatcaga taatagaata cctttaccct
      661 tgcttaatca ttacaccttt ggactgcttc tgggaagggg caaagctaca gtccgggaca
      721 gcatacctcc taggtaagcc tcctttacgg tggacaaact ttgacccctt ggaattccta
      781 gaagagttaa agaaaataaa ctaccaagtg gacagctggg aggaaatgct gaataaagcc
      841 gaagttggcc atgggtacat ggaccggcct tgcctcaacc cagccgaccc agattgccct
      901 gccacagccc ctaacaaaaa ttcaaccaaa cctcttgatg tggcccttgt tttgaatggt
      961 ggatgtcaag gtttatccag gaagtatatg cattggcagg aggagttgat tgtgggtggt
     1021 accgtcaaga atgccactgg aaaacttgtc agcgctcacg ccctgcaaac catgttccag
     1081 ttaatgactc ccaagcaaat gtatgaacac ttcaggggct acgactatgt ctctcacatc
     1141 aactggaatg aagacagggc agccgccatc ctggaggcct ggcagaggac ttacgtggag
     1201 gtggttcatc aaagtgtcgc cccaaactcc actcaaaagg tgcttccctt cacaaccacg
     1261 accctggacg acatcctaaa atccttctct gatgtcagtg tcatccgagt ggccagcggc
     1321 tacctactga tgcttgccta tgcctgttta accatgctgc gctgggactg ctccaagtcc
     1381 cagggtgccg tggggctggc tggcgtcctg ttggttgcgc tgtcagtggc tgcaggattg
     1441 ggcctctgct ccttgattgg catttctttt aatgctgcga caactcaggt tttgccgttt
     1501 cttgctcttg gtgttggtgt ggatgatgtc ttcctcctgg cccatgcatt cagtgaaaca
     1561 ggacagaata agaggattcc atttgaggac aggactgggg agtgcctcaa gcgcaccgga
     1621 gccagcgtgg ccctcacctc catcagcaat gtcaccgcct tcttcatggc cgcattgatc
     1681 cctatccctg ccctgcgagc gttctccctc caggctgctg tggtggtggt attcaatttt
     1741 gctatggttc tgctcatttt tcctgcaatt ctcagcatgg atttatacag acgtgaggac
     1801 agaagattgg atattttctg ctgtttcaca agcccctgtg tcagcagggt gattcaagtt
     1861 gagccacagg cctacacaga gcctcacagt aacacccggt acagcccccc acccccatac
     1921 accagccaca gcttcgccca cgaaacccat atcactatgc agtccaccgt tcagctccgc
     1981 acagagtatg accctcacac gcacgtgtac tacaccaccg ccgagccacg ctctgagatc
     2041 tctgtacagc ctgttaccgt cacccaggac aacctcagct gtcagagtcc cgagagcacc
     2101 agctctacca gggacctgct ctcccagttc tcagactcca gcctccactg cctcgagccc
     2161 ccctgcacca agtggacact ctcttcgttt gcagagaagc actatgctcc tttcctcctg
     2221 aaacccaaag ccaaggttgt ggtaatcctt cttttcctgg gcttgctggg ggtcagcctt
     2281 tatgggacca cccgagtgag agacgggctg gacctcacgg acattgttcc ccgggaaacc
     2341 agagaatatg acttcatagc tgcccagttc aagtacttct ctttctacaa catgtatata
     2401 gtcacccaga aagcagacta cccgaatatc cagcacctac tttacgacct tcataagagt
     2461 ttcagcaatg tgaagtatgt catgctggag gagaacaagc aacttcccca aatgtggctg
     2521 cactacttta gagactggct tcaaggactt caggatgcat ttgacagtga ctgggaaact
     2581 gggaggatca tgccaaacaa ttataaaaat ggatcagatg acggggtcct cgcttacaaa
     2641 ctcctggtgc agactggcag ccgagacaag cccatcgaca ttagtcagtt gactaaacag
     2701 cgtctggtag acgcagatgg catcattaat ccgagcgctt tctacatcta cctgaccgct
     2761 tgggtcagca acgaccctgt agcttacgct gcctcccagg ccaacatccg gcctcaccgg
     2821 ccggagtggg tccatgacaa agccgactac atgccagaga ccaggctgag aatcccagca
     2881 gcagagccca tcgagtacgc tcagttccct ttctacctca acggcctacg agacacctca
     2941 gactttgtgg aagccataga aaaagtgaga gtcatctgta acaactatac gagcctggga
     3001 ctgtccagct accccaatgg ctaccccttc ctgttctggg agcaatacat cagcctgcgc
     3061 cactggctgc tgctatccat cagcgtggtg ctggcctgca cgtttctagt gtgcgcagtc
     3121 ttcctcctga acccctggac ggccgggatc attgtcatgg tcctggctct gatgaccgtt
     3181 gagctctttg gcatgatggg cctcattggg atcaagctga gtgctgtgcc tgtggtcatc
     3241 ctgattgcat ctgttggcat cggagtggag ttcaccgtcc acgtggcttt ggcctttctg
     3301 acagccattg gggacaagaa ccacagggct atgctcgctc tggagcacat gtttgctccc
     3361 gttctggacg gtgctgtgtc cactctgctg ggtgtactga tgcttgcagg gtccgaattt
     3421 gatttcattg tcagatactt ctttgccgtc ctggccattc tcaccgtctt gggggttctc
     3481 aatggactgg ttctgctgcc tgtcctctta tccttctttg gaccgtgtcc tgaggtgtct
     3541 ccagccaatg gcctaaaccg actgcccact ccttcgcctg agccgcctcc aagtgtcgtc
     3601 cggtttgccg tgcctcctgg tcacacgaac aatgggtctg attcctccga ctcggagtac
     3661 agctctcaga ccacggtgtc tggcatcagt gaggagctca ggcaatacga agcacagcag
     3721 ggtgccggag gccctgccca ccaagtgatt gtggaagcca cagaaaaccc tgtctttgcc
     3781 cggtccactg tggtccatcc ggactccaga catcagcctc ccttgacccc tcggcaacag
     3841 ccccacctgg actctggctc cttgtcccct ggacggcaag gccagcagcc tcgaagggat
     3901 ccccctagag aaggcttgcg gccacccccc tacagaccgc gcagagacgc ttttgaaatt
     3961 tctactgaag ggcattctgg ccctagcaat agggaccgct cagggccccg tggggcccgt
     4021 tctcacaacc ctcggaaccc aacgtccacc gccatgggca gctctgtgcc cagctactgc
     4081 cagcccatca ccactgtgac ggcttctgct tcggtgactg ttgctgtgca tcccccgcct
     4141 ggacctgggc gcaacccccg aggggggccc tgtccaggct atgagagcta ccctgagact
     4201 gatcacgggg tatttgagga tcctcatgtg ccttttcatg tcaggtgtga gaggagggac
     4261 tcaaaggtgg aggtcataga gctacaggac gtggaatgtg aggagaggcc gtgggggagc
     4321 agctccaacg ggagaggcag cgggagtggc agtcgcggag gcagtggtgg accggtcgcc
     4381 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     4441 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     4501 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     4561 accctcgtga ccaccttgac ctacggcgtg cagtgcttcg cccgctaccc cgaccacatg
     4621 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     4681 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     4741 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     4801 cacaagctgg agtacaacta caacagccac aaggtctata tcaccgccga caagcagaag
     4861 aacggcatca aggtgaactt caagacccgc cacaacatcg aggacggcag cgtgcagctc
     4921 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     4981 cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg
     5041 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     5101 taagcggccg cgactctaga tcataatcag cca
//
