LOCUS       sequence-84364-e        2527 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Paszek MJ, DuFort CC, Rubashkin MG, Davidson MW, Thorn KS, Liphardt 
            JT, Weaver VM
  TITLE     Scanning angle interference microscopy reveals cell dynamics at the 
            nanoscale.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):825-7. doi: 10.1038/nmeth.2077.
  PUBMED    22751201
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "825-7"
COMMENT     Sequence Label: mEmerald-Paxillin-22
FEATURES             Location/Qualifiers
     source          1..2527
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1699..1751
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1759..1768
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1765..1767,1768..1770,1771..2484)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1810..1831)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2071..2090)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2418..2439
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcac catggacgac ctcgatgcct tactggcaga cctggaatcc
       61 accacctcgc atatctctaa gcgaccggtg tttctaacag aggaaacgcc ttactcctac
      121 ccaactggaa accatacgta ccaggagatt gctgtgccac cgccggtgcc cccgccgcct
      181 tccagtgagg ccctaaatgg cactgtgatt gaccccttag accagtggca acccagcgtg
      241 tccagatacg gccaccagca acctcagtcc cagtctccga tatacagctc tagtgccaaa
      301 agctccagtg cctctgttcc tagagacggg ctcagctccc cttctccccg tgccagtgag
      361 gaggaacacg tgtacagctt cccaaacaag cagaagtctg cagaaccatc tcccacaatg
      421 accagcacct ccctgggcag caacctctcg gaactggaca ggcttctcct ggaactgaat
      481 gctgttcaac ataatccccc cagtggcttc tcagcagatg aagtcagcag aagcccatca
      541 ctgcccaatg tgaccggacc tcactatgtc atcccagaga gcagcagctc tgcaggaggg
      601 aaggctgcac cccctacaaa agaaaaacca aagcggaatg gtgggcgtgg gattgaagat
      661 gtgcgtccca gtgtggagag cctgctggat gagctggaga gctctgtgcc aagcccagtc
      721 cctgcaatca ctgtgagcca aggggaggtg agcagccccc agcgtgtcaa cgccagtcag
      781 cagcagaccc gtatctctgc ttcttcagct acacgagaac tggatgagct gatggcgtcc
      841 ctctctgact ttaagttcat ggcacagggg aaagccggcg gcagcagctc cccaccatcc
      901 accaccccaa agcctggcag tcagctggac accatgctgg gaagtctgca gtctgacctg
      961 aacaaactgg gggtagcgac ggtcgccaag ggtgtgtgcg gagcctgcaa gaagccgatt
     1021 gctgggcagg tagttacagc catggggaag acctggcacc ctgagcactt cgtctgcacc
     1081 cactgccagg aggagattgg atcacggaac ttctttgagc gggatgggca gccctactgt
     1141 gagaaggact atcacaacct cttctctcct cgctgctact actgcaatgg gccaatcctt
     1201 gataaagtgg tgacggcttt ggacaggacg tggcaccctg aacacttttt ctgtgcccag
     1261 tgtggagttt tctttggacc tgaaggattt catgagaagg atggcaaagc ctattgccgc
     1321 aaggactact ttgacatgtt tgctcccaag tgtggaggct gtgcccgggc tatcctggaa
     1381 aattacattt ctgccttaaa caccctgtgg caccctgagt gctttgtctg tcgggaatgt
     1441 ttcacaccgt tcatcaacgg cagcttcttc gagcacgacg gccagcccta ctgcgaggtg
     1501 cattaccacg agcgccgcgg ctcgctgtgc tctggctgcc agaagcccat cacgggacgc
     1561 tgcatcactg ccatgggcaa gaaatttcac cccgaacact ttgtctgtgc cttctgcctc
     1621 aagcagctca acaaaggaac cttcaaagaa cagaacgaca agccctactg tcagaactgc
     1681 tttctcaaac tcttctgtct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg
     1741 gcccgggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     1801 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1861 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     1921 aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt gcagtgcttc
     1981 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     2041 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     2101 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     2161 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caaggtctat
     2221 atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg ccacaacatc
     2281 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     2341 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc
     2401 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     2461 ggcatggacg agctgtacaa gtaagcggcc gcgactctag atcataatca gccataccac
     2521 attgaga
//
