LOCUS       sequence-84366-e        1633 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Lu J, Li F, Murphy CS, Davidson MW, Gilbert DM
  TITLE     G2 phase chromatin lacks determinants of replication timing.
  JOURNAL   J Cell Biol. 2010 Jun 14;189(6):967-80. doi: 10.1083/jcb.201002002. 
            Epub 2010 Jun 7.
  PUBMED    20530209
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201002002"; journalName: "J Cell Biol"; date: 
            "2010-06-14- 14"; volume: "189"; issue: "6"; pages: "967-80"
COMMENT     Sequence Label: mEmerald-PCNA-19
FEATURES             Location/Qualifiers
     source          1..1633
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggagaa gggcaagggc aagggcaagg
      781 gccgggccgc ggctacgcgt atcgatccat gttcgaggcg cgcctggtcc agggctccat
      841 cctcaagaag gtgttggagg cactcaagga cctcatcaac gaggcctgct gggatattag
      901 ctccagcggt gtaaacctgc agagcatgga ctcgtcccac gtctctttgg tgcagctcac
      961 cctgcggtct gagggcttcg acacctaccg ctgcgaccgc aacctggcca tgggcgtgaa
     1021 cctcaccagt atgtccaaaa tactaaaatg cgccggcaat gaagatatca ttacactaag
     1081 ggccgaagat aacgcggata ccttggcgct agtatttgaa gcaccaaacc aggagaaagt
     1141 ttcagactat gaaatgaagt tgatggattt agatgttgaa caacttggaa ttccagaaca
     1201 ggagtacagc tgtgtagtaa agatgccttc tggtgaattt gcacgtatat gccgagatct
     1261 cagccatatt ggagatgctg ttgtaatttc ctgtgcaaaa gacggagtga aattttctgc
     1321 aagtggagaa cttggaaatg gaaacattaa attgtcacag acaagtaatg tcgataaaga
     1381 ggaggaagct gttaccatag agatgaatga accagttcaa ctaacttttg cactgaggta
     1441 cctgaacttc tttacaaaag ccactccact ctcttcaacg gtgacactca gtatgtctgc
     1501 agatgtaccc cttgttgtag agtataaaat tgcggatatg ggacacttaa aatactactt
     1561 ggctcccaag atcgaggatg aagaaggatc ttagggatcc accggatcta gataactgat
     1621 cataatcagc cat
//
