LOCUS       sequence-84367-e        1682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Lu J, Li F, Murphy CS, Davidson MW, Gilbert DM
  TITLE     G2 phase chromatin lacks determinants of replication timing.
  JOURNAL   J Cell Biol. 2010 Jun 14;189(6):967-80. doi: 10.1083/jcb.201002002. 
            Epub 2010 Jun 7.
  PUBMED    20530209
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201002002"; journalName: "J Cell Biol"; date: 
            "2010-06-14- 14"; volume: "189"; issue: "6"; pages: "967-80"
COMMENT     Sequence Label: mEmerald-PCNA-19-NLS-4
FEATURES             Location/Qualifiers
     source          1..1682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             41..61
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      74..83
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(80..82,83..85,86..796)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(125..146)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(386..405)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     733..754
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acggtcagat ccgctagcca ccatggcttc gtggggctcc ccgaagaaga agcgcaaagt
       61 actggtaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      121 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      181 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      241 gcccgtgccc tggcccaccc tcgtgaccac cttgacctac ggcgtgcagt gcttcgcccg
      301 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      361 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      421 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      481 cggcaacatc ctggggcaca agctggagta caactacaac agccacaagg tctatatcac
      541 cgccgacaag cagaagaacg gcatcaaggt gaacttcaag acccgccaca acatcgagga
      601 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      661 gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga
      721 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
      781 ggacgagctg tacaagtccg gagaagggca agggcaaggg caagggccgg gccgcggcta
      841 cgcgtatcga tccatgttcg aggcgcgcct ggtccagggc tccatcctca agaaggtgtt
      901 ggaggcactc aaggacctca tcaacgaggc ctgctgggat attagctcca gcggtgtaaa
      961 cctgcagagc atggactcgt cccacgtctc tttggtgcag ctcaccctgc ggtctgaggg
     1021 cttcgacacc taccgctgcg accgcaacct ggccatgggc gtgaacctca ccagtatgtc
     1081 caaaatacta aaatgcgccg gcaatgaaga tatcattaca ctaagggccg aagataacgc
     1141 ggataccttg gcgctagtat ttgaagcacc aaaccaggag aaagtttcag actatgaaat
     1201 gaagttgatg gatttagatg ttgaacaact tggaattcca gaacaggagt acagctgtgt
     1261 agtaaagatg ccttctggtg aatttgcacg tatatgccga gatctcagcc atattggaga
     1321 tgctgttgta atttcctgtg caaaagacgg agtgaaattt tctgcaagtg gagaacttgg
     1381 aaatggaaac attaaattgt cacagacaag taatgtcgat aaagaggagg aagctgttac
     1441 catagagatg aatgaaccag ttcaactaac ttttgcactg aggtacctga acttctttac
     1501 aaaagccact ccactctctt caacggtgac actcagtatg tctgcagatg taccccttgt
     1561 tgtagagtat aaaattgcgg atatgggaca cttaaaatac tacttggctc ccaagatcga
     1621 ggatgaagaa ggatcttagg gatccaccgg atctagataa ctgatcataa tcagccatac
     1681 ca
//
