LOCUS       sequence-84368-e        1716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Lu J, Li F, Murphy CS, Davidson MW, Gilbert DM
  TITLE     G2 phase chromatin lacks determinants of replication timing.
  JOURNAL   J Cell Biol. 2010 Jun 14;189(6):967-80. doi: 10.1083/jcb.201002002. 
            Epub 2010 Jun 7.
  PUBMED    20530209
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201002002"; journalName: "J Cell Biol"; date: 
            "2010-06-14- 14"; volume: "189"; issue: "6"; pages: "967-80"
COMMENT     Sequence Label: mEmerald-PCNA-19-SV40NLS-4
FEATURES             Location/Qualifiers
     source          1..1716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..48
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             52..72
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      103..112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(109..111,112..114,115..825)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(154..175)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(415..434)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     762..783
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagccac catggatcca aaaaagaaga gaaaggtaga tccaaaaaag
       61 aagagaaagg tagatccaaa aaagaagaga aaggtaccgg tcgccaccat ggtgagcaag
      121 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      181 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      241 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      301 ttgacctacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
      361 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      421 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      481 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      541 aactacaaca gccacaaggt ctatatcacc gccgacaagc agaagaacgg catcaaggtg
      601 aacttcaaga cccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
      661 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
      721 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      781 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg agaagggcaa
      841 gggcaagggc aagggccggg ccgcggctac gcgtatcgat ccatgttcga ggcgcgcctg
      901 gtccagggct ccatcctcaa gaaggtgttg gaggcactca aggacctcat caacgaggcc
      961 tgctgggata ttagctccag cggtgtaaac ctgcagagca tggactcgtc ccacgtctct
     1021 ttggtgcagc tcaccctgcg gtctgagggc ttcgacacct accgctgcga ccgcaacctg
     1081 gccatgggcg tgaacctcac cagtatgtcc aaaatactaa aatgcgccgg caatgaagat
     1141 atcattacac taagggccga agataacgcg gataccttgg cgctagtatt tgaagcacca
     1201 aaccaggaga aagtttcaga ctatgaaatg aagttgatgg atttagatgt tgaacaactt
     1261 ggaattccag aacaggagta cagctgtgta gtaaagatgc cttctggtga atttgcacgt
     1321 atatgccgag atctcagcca tattggagat gctgttgtaa tttcctgtgc aaaagacgga
     1381 gtgaaatttt ctgcaagtgg agaacttgga aatggaaaca ttaaattgtc acagacaagt
     1441 aatgtcgata aagaggagga agctgttacc atagagatga atgaaccagt tcaactaact
     1501 tttgcactga ggtacctgaa cttctttaca aaagccactc cactctcttc aacggtgaca
     1561 ctcagtatgt ctgcagatgt accccttgtt gtagagtata aaattgcgga tatgggacac
     1621 ttaaaatact acttggctcc caagatcgag gatgaagaag gatcttaggg atccaccgga
     1681 tctagataac tgatcataat cagccatacc acattt
//
