LOCUS       sequence-84369-e        1988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1988)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-PDHA1-C-10
FEATURES             Location/Qualifiers
     source          1..1988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(24..26,27..29,30..740)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(69..90)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(330..349)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     677..698
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
       61 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
      121 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
      181 agctgcccgt gccctggccc accctcgtga ccaccttgac ctacggcgtg cagtgcttcg
      241 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
      301 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
      361 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
      421 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aaggtctata
      481 tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc cacaacatcg
      541 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
      601 ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca
      661 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
      721 gcatggacga gctgtacaag tccggaagcg gctccgggag cggaagcggg aggaagatgc
      781 tcgccgccgt ctcccgcgtg ctgtctggcg cttctcagaa gccggcaagc agagtgctgg
      841 tagcatcccg taattttgca aatgatgcta catttgaaat taagaaatgt gaccttcacc
      901 ggctggaaga aggccctcct gtcacaacag tgctcaccag ggaggatggg ctcaaatact
      961 acaggatgat gcagactgta cgccgaatgg agttgaaagc agatcagctg tataaacaga
     1021 aaattattcg tggtttctgt cacttgtgtg atggtcagga agcttgctgt gtgggcctgg
     1081 aggccggcat caaccccaca gaccatctca tcacagccta ccgggctcac ggctttactt
     1141 tcacccgggg cctttccgtc cgagaaattc tcgcagagct tacaggacga aaaggaggtt
     1201 gtgctaaagg gaaaggagga tcgatgcaca tgtatgccaa gaacttctac gggggcaatg
     1261 gcatcgtggg agcgcaggtg cccctgggcg ctgggattgc tctagcctgt aagtataatg
     1321 gaaaagatga ggtctgcctg actttatatg gcgatggtgc tgctaaccag ggccagatat
     1381 tcgaagctta caacatggca gctttgtgga aattaccttg tattttcatc tgtgagaata
     1441 atcgctatgg aatgggaacg tctgttgaga gagcggcagc cagcactgat tactacaaga
     1501 gaggcgattt cattcctggg ctgagagtgg atggaatgga tatcctgtgc gtccgagagg
     1561 caacaaggtt tgctgctgcc tattgtagat ctgggaaggg gcccatcctg atggagctgc
     1621 agacttaccg ttaccacgga cacagtatga gtgaccctgg agtcagttac cgtacacgag
     1681 aagaaattca ggaagtaaga agtaagagtg accctattat gcttctcaag gacaggatgg
     1741 tgaacagcaa tcttgccagt gtggaagaac taaaggaaat tgatgtggaa gtgaggaagg
     1801 agattgagga tgctgcccag tttgccacgg ccgatcctga gccacctttg gaagagctgg
     1861 gctaccacat ctactccagc gacccacctt ttgaagttcg tggtgccaat cagtggatca
     1921 agtttaagtc agtcagttaa ggatccaccg gatctagata actgatcata atcagccata
     1981 ccacattt
//
