LOCUS       sequence-84370-e        1965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Planchon TA, Gao L, Milkie DE, Davidson MW, Galbraith JA, Galbraith 
            CG, Betzig E
  TITLE     Rapid three-dimensional isotropic imaging of living cells using 
            Bessel beam plane illumination.
  JOURNAL   Nat Methods. 2011 May;8(5):417-23. doi: 10.1038/nmeth.1586. Epub 
            2011 Mar 4.
  PUBMED    21378978
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1586"; journalName: "Nat Methods"; date: "2011-05"; 
            volume: "8"; issue: "5"; pages: "417-23"
COMMENT     Sequence Label: mEmerald-PDHA1-N-10
FEATURES             Location/Qualifiers
     source          1..1965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1219..1228
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1225..1227,1228..1230,1231..1944)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1270..1291)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1531..1550)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1878..1899
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagatccg ctagcaccgc caccatgagg aagatgctcg ccgccgtctc ccgcgtgctg
       61 tctggcgctt ctcagaagcc ggcaagcaga gtgctggtag catcccgtaa ttttgcaaat
      121 gatgctacat ttgaaattaa gaaatgtgac cttcaccggc tggaagaagg ccctcctgtc
      181 acaacagtgc tcaccaggga ggatgggctc aaatactaca ggatgatgca gactgtacgc
      241 cgaatggagt tgaaagcaga tcagctgtat aaacagaaaa ttattcgtgg tttctgtcac
      301 ttgtgtgatg gtcaggaagc ttgctgtgtg ggcctggagg ccggcatcaa ccccacagac
      361 catctcatca cagcctaccg ggctcacggc tttactttca cccggggcct ttccgtccga
      421 gaaattctcg cagagcttac aggacgaaaa ggaggttgtg ctaaagggaa aggaggatcg
      481 atgcacatgt atgccaagaa cttctacggg ggcaatggca tcgtgggagc gcaggtgccc
      541 ctgggcgctg ggattgctct agcctgtaag tataatggaa aagatgaggt ctgcctgact
      601 ttatatggcg atggtgctgc taaccagggc cagatattcg aagcttacaa catggcagct
      661 ttgtggaaat taccttgtat tttcatctgt gagaataatc gctatggaat gggaacgtct
      721 gttgagagag cggcagccag cactgattac tacaagagag gcgatttcat tcctgggctg
      781 agagtggatg gaatggatat cctgtgcgtc cgagaggcaa caaggtttgc tgctgcctat
      841 tgtagatctg ggaaggggcc catcctgatg gagctgcaga cttaccgtta ccacggacac
      901 agtatgagtg accctggagt cagttaccgt acacgagaag aaattcagga agtaagaagt
      961 aagagtgacc ctattatgct tctcaaggac aggatggtga acagcaatct tgccagtgtg
     1021 gaagaactaa aggaaattga tgtggaagtg aggaaggaga ttgaggatgc tgcccagttt
     1081 gccacggccg atcctgagcc acctttggaa gagctgggct accacatcta ctccagcgac
     1141 ccaccttttg aagttcgtgg tgccaatcag tggatcaagt ttaagtcagt cagtagctcc
     1201 ggaggggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     1261 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1321 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     1381 aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt gcagtgcttc
     1441 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1501 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1561 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1621 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caaggtctat
     1681 atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg ccacaacatc
     1741 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1801 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc
     1861 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1921 ggcatggacg agctgtacaa gtaagcggcc gcgactctag atcat
//
