LOCUS       sequence-84371-e        2228 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2228)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Peripherin-C-14
FEATURES             Location/Qualifiers
     source          1..2228
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     681..702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc
      241 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagtccgga agtgctggga gcgcaagcgg aagcggaggc
      781 tccggtagcc accacccgtc gggcctccgg gccggcttca gctccacctc ataccgccgt
      841 accttcggtc caccgccctc actatccccc ggggccttct cctactcgtc cagctcccgc
      901 ttctccagca gccgcctgct gggctccgcg tccccgagct cctcggtgcg cctgggcagc
      961 ttccgtagcc cccgagcggg agcgggcgcc ctcctgcgcc tgccctcgga gcgcctcgac
     1021 ttctccatgg ccgaggccct caaccaggag ttcctggcca cgcgcagcaa cgagaagcag
     1081 gagctgcagg agctcaacga ccgcttcgcc aacttcatcg agaaggtacg ctttctggag
     1141 cagcagaacg cggccctgcg cggggagctg agccaagccc ggggccagga gccggcgcgc
     1201 gccgaccagc tgtgccagca ggagctgcgc gagctgcggc gagagctgga gctgttgggc
     1261 cgcgagcgtg accgggtgca ggtggagcgc gacgggctgg cggaggacct ggcggcgctc
     1321 aagcagaggt tggaggagga gacgcgcaag cgggaggacg cggagcacaa cctcgtgctc
     1381 ttccgcaagg acgtggacga tgccactctg tcccgcctgg aactagagcg caagattgag
     1441 tctctgatgg atgagattga gttcctcaag aagctgcacg aggaggagct gcgagacctg
     1501 caggtgagtg tggagagcca gcaggtgcag caggtggagg tggaagccac ggtgaagccc
     1561 gagctgacgg cagcgctgag ggacatccgc gcgcagtacg agagcatcgc cgcgaagaac
     1621 ctgcaggagg cggaggagtg gtacaagtcc aagtacgcgg acctgtccga cgctgccaac
     1681 cggaaccacg aggccctgcg ccaggccaag caggagatga acgagtcccg acgccagatc
     1741 cagagtctaa cgtgcgaggt ggacgggctg cgcggcacga acgaggcgct gctcaggcag
     1801 ttgagagagc tggaggagca gttcgccctg gaggcggggg gctaccaggc gggcgctgcg
     1861 cggctcgagg aggagctgcg acagctaaaa gaggagatgg cgcggcacct gagggagtac
     1921 caggagctcc tcaacgtcaa gatggccctg gacatcgaga tcgccaccta ccgcaagctg
     1981 ctggagggcg aggagagccg gatctccgtg cccgtccatt cttttgcctc cttaaatata
     2041 aagacgactg tgcctgaggt ggagcctccc caggacagcc acagccggaa gacggttctg
     2101 atcaagacca ttgagacccg gaatggggag gtggtgacag agtcccagaa ggagcagcgc
     2161 agtgagctgg acaagtcttc tgcccacagt tactgaggat ccaccggatc tagataactg
     2221 atcataat
//
