LOCUS       sequence-84373-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Peroxisomes-2
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    16..96
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      98..107
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(104..106,107..109,110..820)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(149..170)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(410..429)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     757..778
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgaaccgtca gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattct
       61 gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga
      121 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      181 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      241 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttgac
      301 ctacggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
      361 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      421 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      481 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
      541 caacagccac aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt
      601 caagacccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
      661 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
      721 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
      781 cgccgccggg atcactctcg gcatggacga gctgtacaag tccggatcca agctgtagcg
      841 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
      901 aacctcccac acctccccct gaa
//
