LOCUS       sequence-84374-e        1812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1812)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-PINCH-C-14
FEATURES             Location/Qualifiers
     source          1..1812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1388..1399
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctggca gcggtggagg
      781 cagcgcatcc ggcgccaacg ccctggccag cgccacttgc gagcgctgca agggcggctt
      841 tgcgcccgct gagaagatcg tgaacagtaa tggggagctg taccatgagc agtgtttcgt
      901 gtgcgctcag tgcttccagc agttcccaga aggactcttc tatgagtttg aaggaagaaa
      961 gtactgtgaa catgactttc agatgctctt tgccccttgc tgtcatcagt gtggtgaatt
     1021 caccattggc cgagttatca aagccatgaa taacagctgg catccggagt gcttccgctg
     1081 tgacctctgc caggaagttc tggcagatat cgggtttgtc aagaatgctg ggagacacct
     1141 gtgtcgcccc tgtcataatc gtgagaaagc cagaggcctt gggaaataca tctgccagaa
     1201 atgccatgct atcatcgatg agcagcctct gatattcaag aacgacccct accatccaga
     1261 ccatttcaac tgcgccaact gcgggaagga gctgactgcc gatgcacggg agctgaaagg
     1321 ggagctatac tgcctcccat gccatgataa aatgggggtc cccatctgtg gtgcttgccg
     1381 acggcccatc gaagggcgcg tggtgaacgc tatgggcaag cagtggcatg tggagcattt
     1441 tgtttgtgcc aagtgtgaga aaccctttct tggacatcgc cattatgaga ggaaaggcct
     1501 ggcatattgt gaaactcact ataaccagct atttggtgat gtttgcttcc actgcaatcg
     1561 tgttatagaa ggtggtgtgg tctctgctct taataaggcc tggtgcgtga actgctttgc
     1621 ctgttctacc tgcaacacta aattaacact caagaataag tttgtggagt ttgacatgaa
     1681 gccagtctgt aagaagtgct atgagaaatt tccattggag ctgaagaaaa gacttaagaa
     1741 actagctgag accttaggaa ggaaataagg atccaccgga tctagataac tgatcataat
     1801 cagccatacc ac
//
