LOCUS       sequence-84375-e        1796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1796)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-PINCH-N-14
FEATURES             Location/Qualifiers
     source          1..1796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             621..632
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      1035..1044
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1041..1043,1044..1046,1047..1760)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1086..1107)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1347..1366)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1694..1715
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgcc accatggcca acgccctggc cagcgccact tgcgagcgct
       61 gcaagggcgg ctttgcgccc gctgagaaga tcgtgaacag taatggggag ctgtaccatg
      121 agcagtgttt cgtgtgcgct cagtgcttcc agcagttccc agaaggactc ttctatgagt
      181 ttgaaggaag aaagtactgt gaacatgact ttcagatgct ctttgcccct tgctgtcatc
      241 agtgtggtga attcaccatt ggccgagtta tcaaagccat gaataacagc tggcatccgg
      301 agtgcttccg ctgtgacctc tgccaggaag ttctggcaga tatcgggttt gtcaagaatg
      361 ctgggagaca cctgtgtcgc ccctgtcata atcgtgagaa agccagaggc cttgggaaat
      421 acatctgcca gaaatgccat gctatcatcg atgagcagcc tctgatattc aagaacgacc
      481 cctaccatcc agaccatttc aactgcgcca actgcgggaa ggagctgact gccgatgcac
      541 gggagctgaa aggggagcta tactgcctcc catgccatga taaaatgggg gtccccatct
      601 gtggtgcttg ccgacggccc atcgaagggc gcgtggtgaa cgctatgggc aagcagtggc
      661 atgtggagca ttttgtttgt gccaagtgtg agaaaccctt tcttggacat cgccattatg
      721 agaggaaagg cctggcatat tgtgaaactc actataacca gctatttggt gatgtttgct
      781 tccactgcaa tcgtgttata gaaggtggtg tggtctctgc tcttaataag gcctggtgcg
      841 tgaactgctt tgcctgttct acctgcaaca ctaaattaac actcaagaat aagtttgtgg
      901 agtttgacat gaagccagtc tgtaagaagt gctatgagaa atttccattg gagctgaaga
      961 aaagacttaa gaaactagct gagaccttag gaaggaaatc cggcggtagc ggcggtagcg
     1021 gggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     1081 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1141 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     1201 tgcccgtgcc ctggcccacc ctcgtgacca ccttgaccta cggcgtgcag tgcttcgccc
     1261 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     1321 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1381 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     1441 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaag gtctatatca
     1501 ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa gacccgccac aacatcgagg
     1561 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     1621 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
     1681 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     1741 tggacgagct gtacaagtaa gcggccgcga ctctagatca taatcagcca taccac
//
